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| Status |
Public on Jan 09, 2010 |
| Title |
Expression data from PSARK::IPT Nicotiana and wildtype plants |
| Platform organism |
Solanum lycopersicum |
| Sample organism |
Nicotiana |
| Experiment type |
Expression profiling by array
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| Summary |
Stress-induced leaf senescence could be delayed in transgenic plants expressing isopentenyltransferase (IPT), an enzyme that catalyzes the rate-limiting step in CK synthesis under the control of SARK, a maturation- and stress-inducible promoter (Rivero et al., 2007). Increased CK production resulted in enhanced drought tolerance of the transgenic PSARK::IPT plants, with minimal yield loss (Rivero et al., 2007). We have used a large-scale expression profiling approach to investigate the effects of PSARK::IPT on gene expression to elucidate the molecular events associated with the CK-induced drought tolerance displayed by the PSARK::IPT plants.
We used tomato genechips to capture the differential transcriptional responses of WT and PSARK::IPT plants under control and water stress conditions
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| Overall design |
Leaf samples from well watered (1000 mL per day) and water stressed (300 mL per day) plants were collected for RNA extraction and hybridization on Affymetrix microarrays after 45 days of stress.
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| Contributor(s) |
Blumwald E, Rivero R |
| Citation(s) |
20871100 |
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| Submission date |
Jan 07, 2010 |
| Last update date |
Dec 24, 2015 |
| Contact name |
Zvi Peleg |
| Organization name |
Hebrew University
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| Department |
Inst. of Plant Sciences
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| Street address |
P.O.Box 12
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| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
| GPL4741 |
[Tomato] Affymetrix Tomato Genome Array |
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| Samples (12)
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| GSM494295 |
leaves WT 300 mL, biological rep1 |
| GSM494296 |
leaves WT 300 mL, biological rep2 |
| GSM494297 |
leaves WT 300 mL, biological rep3 |
| GSM494298 |
leaves PSARK::IPT 1000 mL, biological rep1 |
| GSM494299 |
leaves PSARK::IPT 1000 mL, biological rep2 |
| GSM494300 |
leaves PSARK::IPT 1000 mL, biological rep3 |
| GSM494301 |
leaves PSARK::IPT 300 mL, biological rep1 |
| GSM494302 |
leaves PSARK::IPT 300 mL, biological rep2 |
| GSM494303 |
leaves PSARK::IPT 300 mL, biological rep3 |
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| Relations |
| BioProject |
PRJNA121927 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19787_RAW.tar |
8.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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