|
| Status |
Public on Jan 09, 2010 |
| Title |
leaves WT 300 mL, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Nicotiana leaves
|
| Organism |
Nicotiana |
| Characteristics |
tissue: leaves
genome/variation: wildtype
age: ~ 10 weeks
environmental factor: water stressed
|
| Treatment protocol |
control plants (1000 mL per day water per pot); water deficit (300 mL water per day per pot)
|
| Growth protocol |
3 week old plants were transplanted from growth chamber to greenhouse and water stress treatment imposed for 45 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Tomato Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from Nicotiana leaf RNA hybridized to Affymetrix Tomato Genome Array
|
| Data processing |
The data were analyzed with RMA using quantile normalization and background correction feature using Express Version 1.0.4.
|
| |
|
| Submission date |
Jan 07, 2010 |
| Last update date |
Jan 08, 2010 |
| Contact name |
Zvi Peleg |
| Organization name |
Hebrew University
|
| Department |
Inst. of Plant Sciences
|
| Street address |
P.O.Box 12
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE19787 |
Expression data from PSARK::IPT Nicotiana and wildtype plants |
|
