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| Status |
Public on Jul 31, 2010 |
| Title |
Transcript profiling of tomato mutants producing anthocyanins in the fruit |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by array
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| Summary |
Anthocyanins are high value plant antioxidants which are not present in the fruits of cultivated tomato. However, both the dominant gene Anthocyanin fruit (Aft) and the recessive gene atroviolacea (atv), introgressed into domesticated tomato from two different wild Solanum species, stimulate a limited anthocyanin pigmentation. Surprisingly, double mutant Aft/Aft atv/atv tomatoes are characterised by the presence of anthocyanins in the fruit peel, resulting in intensely purple pigmented fruit.
We carried out a transcript profiling analysis using GeneChip® Tomato Genome Arrays, in order to identify differentially expressed genes when comparing wild type, Aft/Aft, atv/atv, and Aft/Aft atv/atv fruits. The expression pattern of several genes involved in the anthocyanin pathway was analyzed in detail. Among the fruit peel-associated differentially expressed transcripts, genes involved in phenylpropanoid pathway, cell wall composition, biotic and abiotic stress responses, sugar and hormone metabolism were overrepresented in Aft/Aft atv/atv. Transcriptomic analysis thus revealed that the activation of anthocyanin synthesis in tomato fruit was accompanied by a complex remodulation of gene expression, likely affecting important agronomic and merceological traits.
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| Overall design |
Wild type (Cv. Ailsa Craig, accession number LA2838A), Aft/Aft (accession number LA1996), atv/atv (accession number LA0797) and double mutant (Aft/Aft atv/atv) were grown during the winter season in a controlled heated greenhouse. Fruits were collected at mature green, turning red and red stages of development. The transcriptional profile in Aft/Aft, atv/atv, and Aft/Aft atv/atv fruits when compared to the wild type was analyzed using the GeneChip® Tomato Genome Array.
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| Contributor(s) |
Povero G, Gonzali S, Perata P |
| Citation(s) |
20888667 |
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| Submission date |
Jan 07, 2010 |
| Last update date |
Dec 24, 2015 |
| Contact name |
Pierdomenico Perata |
| E-mail(s) |
p.perata@sssup.it
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| Phone |
+39050881901
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| Organization name |
Sant'Anna School of Advanced Studies
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| Department |
Institute of Life Sciences
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| Lab |
PlantLab
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| Street address |
Via Guidiccioni 8-10
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| City |
San Giuliano Terme |
| State/province |
Pisa |
| ZIP/Postal code |
56010 |
| Country |
Italy |
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| Platforms (1) |
| GPL4741 |
[Tomato] Affymetrix Tomato Genome Array |
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| Samples (8)
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| GSM494344 |
wild type (cv. Ailsa Craig), biological rep1 |
| GSM494345 |
wild type (cv. Ailsa Craig), biological rep2 |
| GSM494346 |
Aft (Anthocyanin fruit) mutant, biological rep1 |
| GSM494347 |
Aft (Anthocyanin fruit) mutant, biological rep2 |
| GSM494348 |
atv (atroviolacea) mutant, biological rep1 |
| GSM494349 |
atv (atroviolacea) mutant, biological rep2 |
| GSM494350 |
Aft/Aft atv/atv double mutant, biological rep1 |
| GSM494351 |
Aft/Aft atv/atv double mutant, biological rep2 |
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| Relations |
| BioProject |
PRJNA121815 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19792_RAW.tar |
12.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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