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| Status |
Public on Apr 20, 2022 |
| Title |
Transcriptome analysis of virgin and mated Anopheles gambiae and Anopheles albimanus |
| Organisms |
Anopheles gambiae; Anopheles albimanus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We custom-built a bioinformatics pipeline to search for 20E-modifying enzymes in the accessory glands of Anopheles gambiae males, searching for ecdysteroid kinases (EcK), ecdysone oxidases (EO), and ecdysteroid-phosphate phosphatases (EPP). To this end, we generated RNAseq datasets of different An. gambiae tissues dissected from virgin and mated females and males, and produced similar datasets for Anopheles albimanus, a South American species that does not synthetize and transfer ecdysteroids during mating. These analyses led to the identification of one candidate EPP and two potential EcKs (EcK1 and EcK2), which we demonstrated are involved in the activity of a male-specific oxidized ecdysteroid (3D20E). We further determined that 3D20E is specifically produced by the An. gambiae male accessory glands and is transferred to females during copulation, where it triggers a series of post-mating responses.
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| Overall design |
RNAseq datasets of different Anopheles gambiae and Anopheles albimanus tissues dissected from virgin and mated females and males. Tissues analyzed included: male accessory glands and rest of the body (for males); atria, head, spermatheca and rest of body (for females).
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| Contributor(s) |
Kakani EG, Mitchell SN, Peng D, Shaw WR, Catteruccia F |
| Citation(s) |
35794471 |
| |
| Submission date |
Mar 15, 2022 |
| Last update date |
Aug 16, 2022 |
| Contact name |
Flaminia Catteruccia |
| Organization name |
Harvard University
|
| Street address |
655 huntington ave
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (2) |
| GPL32062 |
Illumina HiSeq 2500 (Anopheles albimanus) |
| GPL32063 |
Illumina HiSeq 2500 (Anopheles gambiae) |
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| Samples (88)
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| Relations |
| BioProject |
PRJNA816430 |
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