|
| Status |
Public on Apr 20, 2022 |
| Title |
BT3_ALBI_At_M |
| Sample type |
SRA |
| |
|
| Source name |
Anopheles albimanus Mated Atria rep B 24 hr
|
| Organism |
Anopheles albimanus |
| Characteristics |
tissue: Atria
Sex: Female
mating status: Mated
replicate: B
time point: 24 hr post-mating or time-matching virgins
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted with TRI reagent (Thermo Fisher Scientific, MA) from pools of 10–12 tissues following the manufacturer’s instructions. RNA was treated with TURBO DNase (Thermo Fisher Scientific, MA), and purified with Qiagen RNeasy MinElute Cleanup Kit.
Illumina TruSeq Stranded mRNA kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Alignment: hisat2/2.0.5. Parameters: -q -x --threads 4
Mapping quality filter: samtools/1.3.1. Parameters: view -bS -q 30
Sort mapped reads: samtools/1.3.1. Parameter: sort
Read count: htseq/0.9.1. Parameters: --stranded=no --mode=union --quiet -a 30
Assembly: Anopheles gambiae PEST AgamP4.12; Anopheles albimanus STECLA AalbS2.4
Supplementary files format and content: Processed data files format: Plain text file. Content: Read counts
|
| |
|
| Submission date |
Mar 15, 2022 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Flaminia Catteruccia |
| Organization name |
Harvard University
|
| Street address |
655 huntington ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL32062 |
| Series (1) |
| GSE198665 |
Transcriptome analysis of virgin and mated Anopheles gambiae and Anopheles albimanus |
|
| Relations |
| BioSample |
SAMN26676102 |
| SRA |
SRX14467854 |
