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| Status |
Public on Mar 25, 2022 |
| Title |
Time-series RNAseq analysis following lateral root induction by gravistimulation |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the comparison of transcriptomic profiles in specific lateral root tissues for Col-0 wild type and puchi-1 mutant seedlings. Lateral root organogenesis is a key process in plant root system development and adaptation to the environment. To dissect the molecular events occurring during the early phase, we generated time-series transcriptomic datasets profiling lateral root development in puchi-1 and wild type backgrounds. Consistent with a mutually inhibitory mechanism, transcriptomic and reporter analysis revealed meristem-related genes were ectopically expressed during early stages of lateral root primordium formation in puchi-1. We conclude that PUCHI participates to the coordination of lateral root patterning and represses ectopic establishment of meristematic cell identities during early stages of organ development.
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| Overall design |
The experiment comprises 36 samples: 2 genotypes (Col-0 wild type and puchi-1 mutant), and 6 treatments (no treatment, 12h, 18h, 24h, 30h and 36h gravistimulation), with 3 replicates per condition (genotype and treatment).
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| Contributor(s) |
BELLANDE K, GUYOMARC'H S, LAPLAZE L, GONZALEZ A, DUBOIS E |
| Citation(s) |
35224628 |
| Submission date |
Mar 22, 2022 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Soazig GUYOMARC'H |
| E-mail(s) |
soazig.guyomarch@ird.fr
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| Organization name |
University of Montpellier
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| Lab |
Plant Diversity, Adaptation and Development (DIADE), IRD
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| Street address |
911 avenue Agropolis
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| City |
Montpellier |
| ZIP/Postal code |
34394 |
| Country |
France |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA818647 |
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