NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE199760 Query DataSets for GSE199760
Status Public on Jan 09, 2023
Title Conserved and plant-specific GCN5-containing complexes cooperate to regulate gene transcription and plant development [RNA-Seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Although the conserved histone acetyltransferase HAG1/GCN5-containing complex SAGA (Spt-Ada-Gcn5 acetyltransferase) has been extensively studied in plants, whether HAG1 forms a plant-specific complex is unknown. Here, we identified a plant-specific GCN5-containing complex, PAGA (plant-ADA2A-GCN5-acetyltransferase), in Arabidopsis thaliana; the complex consists of two conserved subunits (HAG1/GCN5 and ADA2A) and four plant-specific subunits (SPC, ING1, SDRL, and EAF6). In the PAGA complex, SPC functions as a scaffold protein that is responsible for integrating the other subunits. SPC also enhances the binding of ING1 to histone H3 with methylated lysine 4 and promotes the histone acetylation activity of HAG1. Chromatin immunoprecipitation followed by sequencing indicated that PAGA not only shares target genes with SAGA but also has its specific target genes. While PAGA and SAGA promote transcription by mediating moderate and high levels of histone acetylation, respectively, at different sets of genes, PAGA can also function antagonistically against SAGA to prevent excessive transcription of PAGA- and SAGA-shared target genes. Unlike SAGA, which regulates multiple biological processes, PAGA is mainly involved in plant height and branch growth by regulating the transcription of genes involved in hormone biosynthesis and response. These results indicate that the HAG1-containing SAGA and PAGA complexes are coordinated to regulate gene transcription and development.
 
Overall design Examination of the mRNA expression of wild type and nine mutants in Arabidopsis
 
Contributor(s) He X
Citation(s) 36879016
Submission date Mar 30, 2022
Last update date Apr 10, 2023
Contact name xinjian He
E-mail(s) hexinjian@nibs.ac.cn
Organization name National Institute of Biological Sciences
Lab hexinjian
Street address No. 7, zhongguancun life science park road, changping district
City Beijing
ZIP/Postal code 102206
Country China
 
Platforms (1)
GPL26208 Illumina NovaSeq 6000 (Arabidopsis thaliana)
Samples (30)
GSM5983961 Col-0_12d_rep1
GSM5983962 Col-0_12d_rep2
GSM5983963 Col-0_12d_rep3
This SubSeries is part of SuperSeries:
GSE199761 Conserved and plant-specific GCN5-containing complexes cooperate to regulate gene transcription and plant development
Relations
BioProject PRJNA821458

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE199760_gene_expression_12d.txt.gz 6.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.