 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 28, 2010 |
| Title |
Transcriptional Characterization of Human Embryonic Stem Cell-Derived Endothelial Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Differentiation of human embryonic stem cells into endothelial cells (hESC-ECs) has the potential to provide an unlimited source of cells for novel transplantation therapies of ischemic diseases by supporting angiogenesis and vasculogenesis. In this study, we developed an extracellular matrix culture system for increasing endothelial differentiation and free from contaminating animal cells. We investigated the transcriptional changes that occur during endothelial differentiation of hESCs using whole genome microarray, and compared to human umbilical vein endothelial cells (HUVECs). (Note: Please see our publication, PMID 20046878, which includes analysis of array data from GSM348001-GSM348004 in conjunction with this series).
|
| |
|
| Overall design |
Total RNA from Day 12 Embryoid Bodies that were differentiated from H9 human embryonic stem cells (EB, n = 4), endothelial cells differentiated from H9 human embryonic stem cells (hESC-EC, n = 4), and human umbilical vein endothelial cells (HUVECs, n = 4) was hybridized to four Agilent Whole Human Genome microarrays. A pooled reference sample was used that contained total RNA from H9 hESCs, H9 embryoid bodies, cardiac and endothelial cells derived from H9 hESCs, cardiac fetal tissue, and human umbilical vein endothelial cells (HUVECs).
|
| |
|
| Contributor(s) |
Wilson KD, Li Z |
| Citation(s) |
20046878, 24463812 |
| |
| Submission date |
Jan 22, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Kitchener D. Wilson |
| E-mail(s) |
kitchwilson@stanford.edu
|
| Organization name |
Stanford University
|
| Street address |
S140 Grant Bldg
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA124085 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20013_RAW.tar |
248.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...