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Series GSE20014 Query DataSets for GSE20014
Status Public on Feb 01, 2010
Title Minicircle-derived iPS cells - Agilent Data
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem (iPS) cells. We report the use of ‘minicircle’ DNA, a vector type that is free of bacterial DNA and capable of high expression in cells. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells. (Note: Our Nature Methods publication included analysis of array data from GSM374067 and GSM374068 in conjunction with this series).
Overall design Total RNA from H7 hESCs (n = 2) and minicircle-derived human iPS cells (n = 2 subclones from adipose tissue of two individual patients) was hybridized to four Agilent Whole Human Genome microarrays. A pooled reference sample was used that contained total RNA from H9 hESCs, H9 embryoid bodies, cardiac and endothelial cells derived from H9 hESCs, cardiac fetal tissue, and human umbilical vein endothelial cells (HUVECs).
Contributor(s) Wilson KD, Jia F
Citation(s) 20139967
Submission date Jan 22, 2010
Last update date Jan 23, 2019
Contact name Kitchener D. Wilson
Organization name Stanford University
Street address S140 Grant Bldg
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (6)
GSM499755 H7 hESC-1
GSM499756 H7 hESC-2
GSM499757 mc-iPSC-1s
BioProject PRJNA124087

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20014_RAW.tar 32.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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