|
| Status |
Public on Apr 13, 2024 |
| Title |
m6a methylation drives IMP1 regulation during human neuronal differentiation [iCLiP] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Human neurodevelopment requires differentiating neurons to establish large networks of connections in a highly stereotyped manner. Neuronal differentiation in particular requires RNA-binding proteins to spatiotemporally regulate thousands of different mRNAs. Yet how these proteins precisely relate to neuronal development and coordinate the expression of functionally coherent genes in a cell type specific manner is only partially understood. To address this, we sought to clarify how the paradigmatic RNA-binding protein IMP1/IGF2BP1, an essential developmental factor, selects and regulates its RNA targets transcriptome-wide during the differentiation of human pluripotent stem cell-derived neural precursor cells to their neuronal counterparts. We used a combination of systemic and molecular analyses to show that IMP1 directly binds to and regulates the expression of a large sets of mRNAs.
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| |
|
| Overall design |
iCLIP on IPSC-derived NPC and neurons for IMP1
|
| |
|
| Contributor(s) |
Petrić Howe M, Harley J, Crook H, Serra SE, Chakrabati AM, Luisier R, Patani R, Ramos A, Klein P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 23, 2022 |
| Last update date |
Apr 15, 2024 |
| Contact name |
Pierre Klein |
| E-mail(s) |
klein.pierre1@gmail.com
|
| Organization name |
University College London
|
| Department |
Institute of Structural and Molecular Biology
|
| Street address |
Gower Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6XA |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (19)
|
| GSM6176379 |
NPC_IMP1_iCLIP_tech_replicate_1_bio_rep1 |
| GSM6176380 |
NPC_IMP1_iCLIP_tech_replicate_2_bio_rep1 |
| GSM6176381 |
NPC_IMP1_iCLIP_tech_replicate_3_bio_rep1 |
| GSM6176382 |
NPC_IMP1_iCLIP_tech_replicate_1_bio_rep2 |
| GSM6176383 |
NPC_IMP1_iCLIP_tech_replicate_2_bio_rep2 |
| GSM6176384 |
NPC_IMP1_iCLIP_tech_replicate_3_bio_rep2 |
| GSM6176385 |
NPC_IMP1_iCLIP_tech_replicate_1_bio_rep3 |
| GSM6176386 |
NPC_IMP1_iCLIP_tech_replicate_2_bio_rep3 |
| GSM6176387 |
NPC_IMP1_iCLIP_tech_replicate_3_bio_rep3 |
| GSM6176388 |
Neuron_IMP1_iCLIP_tech_replicate_1_bio_rep1 |
| GSM6176389 |
Neuron_IMP1_iCLIP_tech_replicate_2_bio_rep1 |
| GSM6176390 |
Neuron_IMP1_iCLIP_tech_replicate_3_bio_rep1 |
| GSM6176391 |
Neuron_IMP1_iCLIP_tech_replicate_1_bio_rep2 |
| GSM6176392 |
Neuron_IMP1_iCLIP_tech_replicate_2_bio_rep2 |
| GSM6176393 |
Neuron_IMP1_iCLIP_tech_replicate_3_bio_rep2 |
| GSM6176394 |
Neuron_IMP1_iCLIP_tech_replicate_1_bio_rep3 |
| GSM6176395 |
Neuron_IMP1_iCLIP_tech_replicate_2_bio_rep3 |
| GSM6176396 |
Neuron_IMP1_iCLIP_tech_replicate_3_bio_rep3 |
| GSM6176397 |
IgG_iCLIP |
|
| This SubSeries is part of SuperSeries: |
| GSE203571 |
m6a methylation drives IMP1 regulation during human neuronal differentiation. |
|
| Relations |
| BioProject |
PRJNA841467 |
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