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Status |
Public on Jun 05, 2022 |
Title |
Functional Immunophenotyping of Children with Critical Status Asthmaticus Identifies Differential Gene Expression Responses in Neutrophils Exposed to a Poly(I:C) Stimulus |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We determined whether we could identify clusters of children with critical asthma by functional immunophenotyping using an intracellular viral analog stimulus. We performed a single-center, prospective, observational cohort study of 43 children ages 6 – 17 years admitted to a pediatric intensive care unit for an asthma attack between July 2019 to February 2021.
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Overall design |
Neutrophils were isolated from children, stimulated overnight with LyoVec poly(I:C), and mRNA was analyzed using a targeted Nanostring immunology array. Network analysis of the differentially expressed transcripts for the paired LyoVec poly(I:C) samples was performed.
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Contributor(s) |
Grunwell JR, Kamaleswaran R |
Citation(s) |
36385161 |
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Submission date |
May 31, 2022 |
Last update date |
Nov 29, 2022 |
Contact name |
Milad Rad |
Organization name |
Georgia Institute of Technology
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Department |
School of Electrical and Computer Engineering
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Street address |
266 Ferst Dr NW
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City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30332 |
Country |
USA |
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Platforms (1) |
GPL21847 |
nCounter Nanostring Human Immunology v2 (Catalog #115000061) |
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Samples (144)
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Relations |
BioProject |
PRJNA844008 |