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| Status |
Public on Apr 27, 2010 |
| Title |
Knockdown of PHF8 in HeLa S3 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
PHF8 is an H3K9me2 demethylase, interacts with H3K4me3 and RNA Polymerase II, is enriched at thousands of transcription start sites and can act as a transcriptional co-activator.
In the following experiment we wanted to analyze the impact of PHF8-knockdown on transcript levels in HeLa cells.
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| Overall design |
Three independent replicate knockdown experiments with PHF8-shA and control NT-sh were performed (6 samples in total).
The two samples of each replicate experiment were hybridized in two color dye swap experiments (6 arrays in total).
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| Contributor(s) |
Fortschegger K, Shiekhattar R |
| Citation(s) |
20421419 |
| |
| Submission date |
Mar 01, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Klaus Fortschegger |
| Organization name |
Center for Genomic Regulation
|
| Department |
Gene Regulation
|
| Lab |
Ramin Shiekhattar
|
| Street address |
C/ Dr. Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE20753 |
PHF8 targets histone methylation and RNA polymerase II to activate transcription |
|
| Relations |
| BioProject |
PRJNA129861 |
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