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| Status |
Public on Jul 16, 2022 |
| Title |
Genome-wide analysis of gene expression regulated by APPL1/2 proteins in human PC-3U cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Transforming growth factor β (TGFβ) is overexpressed in several advanced cancers and promotes tumor progression. How cancer cells evade TGFβ-induced growth inhibition and escape normal homeostasis is unclear. Here, we report that the TGFβ Type I receptor (TβRI), together with the endosomal adaptor proteins APPL1 and APPL2, promote expression of survivin and Aurora kinase B (AURKB). We show that the intracellular part of TβRI binds to AURKB during telophase in PC-3U prostate cancer and KELLY neuroblastoma cells. The TβRI/tumor necrosis factor receptor–associated factor 6 (TRAF6) complex activates AURKB by causing TRAF6-induced Lys63-linked polyubiquitination of AURKB at Lys85 and Lys87, promoting cancer cell division and survival. This mechanism may contribute to the pro-tumorigenic effects of TGFβ in advanced cancers.
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| Overall design |
Total RNA obtained from PC-3U cells in which APPL1 and APPL2 were knocked down by siRNA, compared to the cells which were transfected with control siRNA. Three replicates per group were included.
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| Contributor(s) |
Song J, Landström M |
| Citation missing |
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| |
| Submission date |
Jun 16, 2022 |
| Last update date |
Jul 18, 2022 |
| Contact name |
Jie Song |
| E-mail(s) |
jie.song@umu.se
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| Phone |
090-785 44 81
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| Organization name |
Umea University
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| Department |
Medical Bioscience
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| Street address |
Medical Biosciences Department, Pathology, M21
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| City |
Umea |
| State/province |
AC |
| ZIP/Postal code |
90185 |
| Country |
Sweden |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA849790 |
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