|
| Status |
Public on Jul 16, 2022 |
| Title |
Cells_APPL1/2siRNA_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
PC-3U cells, APPL1/2 siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PC-3U
tumor stage: grade IV prostatic adenocarcinoma
metastasis: bone
treatement: APPL1/2 siRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from PC-3U cells using the RNeasy Mini Kit (Qiagen). RNA purity and integrity were evaluated with the Agilent RNA 6000 Nano Kit and Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the TargetAmp-Nano Labeling Kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalized using quantile normalization with GenomeStudio
|
| |
|
| Submission date |
Jun 16, 2022 |
| Last update date |
Jul 16, 2022 |
| Contact name |
Jie Song |
| E-mail(s) |
jie.song@umu.se
|
| Phone |
090-785 44 81
|
| Organization name |
Umea University
|
| Department |
Medical Bioscience
|
| Street address |
Medical Biosciences Department, Pathology, M21
|
| City |
Umea |
| State/province |
AC |
| ZIP/Postal code |
90185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE206243 |
Genome-wide analysis of gene expression regulated by APPL1/2 proteins in human PC-3U cells |
|
