 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 13, 2022 |
| Title |
MTA mediated m6A modification promotes chilling tolerance by regulating gene translation efficiency in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Plants can respond to low temperatures with diverse mechanisms occurred at transcriptional and translational levels. N6-methyladenosine (m6A) is the most prevalent mRNA modification in eukaryotes that acts as a new emerging layer of epitranscriptomic gene regulation. However, the cellular roles of m6A in plant response to low temperatures remain largely unknown. Here we show that mRNA m6A modification is indispensable for Arabidopsis growth under chilling conditions.
|
| |
|
| Overall design |
Gene expression,translation efficiency and m6A peaks were identified from wild type Columbia-0 and AmiR-mta mutant under chilling and normal conditions in two biological replicates.
|
| |
|
| Contributor(s) |
Wang S, Wang H, Xu Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 16, 2022 |
| Last update date |
Dec 16, 2022 |
| Contact name |
Wang Haiyan |
| E-mail(s) |
2018201068@njau.edu.cn
|
| Organization name |
Nanjing Agricultural University
|
| Department |
State Key Laboratory for Crop Genetics and Germplasm Enhancement
|
| Lab |
Bioinformatics Center
|
| Street address |
Weigang NO.1
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210095 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
|
| Samples (32)
|
| GSM6249958 |
Col-0,control,input,rep2 |
| GSM6249959 |
Col-0,chilling,m6A-IP,rep1 |
| GSM6249960 |
Col-0,chilling,m6A-IP,rep2 |
| GSM6249961 |
Col-0,chilling,input,rep1 |
| GSM6249962 |
Col-0,chilling,input,rep2 |
| GSM6249963 |
AmiR-mta,control,m6A-IP,rep1 |
| GSM6249964 |
AmiR-mta,control,m6A-IP,rep2 |
| GSM6249965 |
AmiR-mta,control,input,rep1 |
| GSM6249966 |
AmiR-mta,control,input,rep2 |
| GSM6249967 |
AmiR-mta,chilling,m6A-IP,rep1 |
| GSM6249968 |
AmiR-mta,chilling,m6A-IP,rep2 |
| GSM6249969 |
AmiR-mta,chilling,input,rep1 |
| GSM6249970 |
AmiR-mta,chilling,input,rep2 |
| GSM6249971 |
Col-0,control,mRNA-seq,rep1 |
| GSM6249972 |
Col-0,control,mRNA-seq,rep2 |
| GSM6249973 |
Col-0,chilling,mRNA-seq,rep1 |
| GSM6249974 |
Col-0,chilling,mRNA-seq,rep2 |
| GSM6249975 |
AmiR-mta,control,mRNA-seq,rep1 |
| GSM6249976 |
AmiR-mta,control,mRNA-seq,rep2 |
| GSM6249977 |
AmiR-mta,chilling,mRNA-seq,rep1 |
| GSM6249978 |
AmiR-mta,chilling,mRNA-seq,rep2 |
| GSM6249979 |
Col-0,control,polysome_seq,rep1 |
| GSM6249980 |
Col-0,control,polysome_seq,rep2 |
| GSM6249981 |
Col-0,chilling,polysome_seq,rep1 |
| GSM6249982 |
Col-0,chilling,polysome_seq,rep2 |
| GSM6249983 |
AmiR-mta,control,polysome_seq,rep1 |
| GSM6249984 |
AmiR-mta,control,polysome_seq,rep2 |
| GSM6249985 |
AmiR-mta,chilling,polysome_seq,rep1 |
| GSM6249986 |
AmiR-mta,chilling,polysome_seq,rep2 |
|
| Relations |
| BioProject |
PRJNA849964 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206292_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of BW, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...