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| Status |
Public on Jan 30, 2023 |
| Title |
IL-13 stimulation of primary human bronchial epithelial cells in control individuals and patients with asthma. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA sequencing of gene expression of differentiated primary human bronchial epithelial cells derived from control and asthmatic patients, stimulated with IL-13.
The Type 2 Asthma mediator IL-13 was described to induce airway hyperresponsiveness, goblet cell metaplasia, mucus hypersecretion and airway remoddeling including impairment of epithelial barrier integrity.
We investigated differential expression of SARS-CoV-2 related host gene expression as well as genes involved in N-linked glycosylation upon IL-13 in bronchial epithelial cells.
Top IL-13 affected pathways included ion- and transmembrane transport, lipid metabolic processed and protein glycosylation.
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| Overall design |
RNA sequencing of gene expression in differentiated primary human bronchial epithelial cells upon IL-13 treatment in healthy controls and patients with asthma.
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| Contributor(s) |
Paulina W, Ge T |
| Citation(s) |
36642382 |
| Submission date |
Jun 20, 2022 |
| Last update date |
Feb 06, 2023 |
| Contact name |
Milena Sokolowska |
| Organization name |
Swiss Institute of Allergy and Asthma Research (SIAF)
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| Street address |
Herman-Burchard-Strasse-9
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| City |
Davos Wolfgang |
| State/province |
Graubünden |
| ZIP/Postal code |
7265 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (20)
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| GSM6255648 |
B113, IL-13 |
| GSM6255649 |
B90, unstimulated |
| GSM6255650 |
B90, IL-13 |
| GSM6255651 |
B129, unstimulated |
| GSM6255652 |
B129, IL-13 |
| GSM6255653 |
B87, unstimulated |
| GSM6255654 |
B87, IL-13 |
| GSM6255655 |
B117, unstimulated |
| GSM6255656 |
B117, IL-13 |
| GSM6255657 |
B116, unstimulated |
| GSM6255658 |
B116, IL-13 |
| GSM6255659 |
B99, unstimulated |
| GSM6255660 |
B99, IL-13 |
| GSM6255661 |
B111, unstimulated |
| GSM6255662 |
B111, IL-13 |
| GSM6255663 |
B121, unstimulated |
| GSM6255664 |
B121, IL-13 |
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| Relations |
| BioProject |
PRJNA851158 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206510_Asthma_IL13_over_Control_IL13.txt.gz |
3.6 Mb |
(ftp)(http) |
TXT |
| GSE206510_Asthma_IL13_over_unst.txt.gz |
3.6 Mb |
(ftp)(http) |
TXT |
| GSE206510_Control_IL13_over_unst.txt.gz |
3.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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