|
| Status |
Public on Jan 30, 2023 |
| Title |
B87, IL-13 |
| Sample type |
SRA |
| |
|
| Source name |
primary bronchial epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Control
gender: female
cell type: primary bronchial epithelial cells
treatment: IL-13
time: 24h
|
| Treatment protocol |
Upon full differentiation (4 weeks), cells have been treated with IL-13 (50ng/ml) and harvested 24h post stimulation
|
| Growth protocol |
bronchial epithelial cells were maintained in BEGM medium (Lonza), diluted 1:1 with DMEM containing 10% fetal bovine serum (FBS) in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Plus Micro Kit (Qiagen, Hilden, Germany), according to manufacturers protocol.
Samples with RNA integrity greater than 9.0 were chosen for library preparation with the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, California), according to manufacturers protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
High Output v4
Control_IL13_over_unst.txt
Asthma_IL13_over_Control_IL13.txt
|
| Data processing |
Data was processed with the workflow available at https://github.com/uzh/ezRun
Significant treshold was set to FDR > 0.05 calculated using the edgeR R package
Normalized abundance measurements was performed by edgeR
Assembly: GRCh38.p10
Supplementary files format and content: tab-delimited text files include FPKM values and normalized counts for each Sample, including fdr and p-value
|
| |
|
| Submission date |
Jun 20, 2022 |
| Last update date |
Jan 30, 2023 |
| Contact name |
Milena Sokolowska |
| Organization name |
Swiss Institute of Allergy and Asthma Research (SIAF)
|
| Street address |
Herman-Burchard-Strasse-9
|
| City |
Davos Wolfgang |
| State/province |
Graubünden |
| ZIP/Postal code |
7265 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE206510 |
IL-13 stimulation of primary human bronchial epithelial cells in control individuals and patients with asthma. |
|
| Relations |
| BioSample |
SAMN29212316 |
| SRA |
SRX15803865 |
