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| Status |
Public on Jul 01, 2022 |
| Title |
Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans [RNA-Seq] |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The fungus Candida albicans is part of the human microbiome and mainly colonises the GI tract of healthy individuals. However, when the balance in the GI tract is disturbed, the fungus can switch from a commensal to a virulent lifestyle and can turn into a life-threatening pathogen. Life in the host is characterised by a constant struggle for nutrients, essential trace elements such as iron, copper and zinc are particularly important. To protect itself from pathogens, the human host has developed a strategy to limit the availability of trace elements, this strategy is also described as nutritional immunity. C. albicans has developed a whole range of counter-strategies to obtain sufficient trace elements. In this manuscript, we have characterised the transcription factor Orf19.217, which acts at the interface between trace element scavenging and acquisition and is therefore particularly important in adaptation to life in the host. According to its identified function, we named the transcription factor Irf1, Iron-dependent Regulator of Filamentation. The transcription factor was previously identified in a systematic screen of genes whose overexpression contributes to morphological changes in C. albicans. We have comprehensively investigated the function of Irf1 using state-of-the-art functional genomics approaches, including ChIP-seq, transcriptomics and bioinformatics analyses. Analysis of the Irf1 gene-regulatory network indicates that the transcription factor is involved in the regulation of genes important for iron uptake. Using phenotypic analysis, we confirmed that Irf1 acts in a pathway parallel to known iron uptake regulators and is also required for hyphal formation induced by iron deficiency, a process that allows the fungus to access more viable body niches. Through epistasis experiments, we were also able to identify downstream effectors of Irf1 that are involved in this process. Our study provides new insights into how C. albicans adapts to iron deficiency and establish a direct functional link between hyphal formation and iron uptake.
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| Overall design |
RNA-Seq of irf1 mutant strains or IRF1 overexpression strains, in triplicate including respective control strain samples (matching parental strain)
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| Contributor(s) |
van Wijlick L, Znaidi S, Bachellier-Bassi S, d’Enfert C |
| Citation(s) |
36004328 |
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| Submission date |
Jun 28, 2022 |
| Last update date |
Nov 09, 2022 |
| Contact name |
Lasse van Wijlick |
| E-mail(s) |
lasse.vanwijlick@pasteur.fr
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| Organization name |
Institut Pasteur
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| Department |
Mycology
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| Lab |
BPF
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| Street address |
25-28 Rue du Dr Roux
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| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
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| Platforms (1) |
| GPL19036 |
Illumina HiSeq 2500 (Candida albicans) |
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| Samples (18)
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| GSM6276722 |
2h overexpression, TET-promoter, IRF1, 1 |
| GSM6276723 |
2h overexpression, TET-promoter, IRF1, 2 |
| GSM6276724 |
2h overexpression, TET-promoter, IRF1, 3 |
| GSM6276725 |
2h control_sample_1 |
| GSM6276726 |
2h control_sample_2 |
| GSM6276727 |
2h control_sample_3 |
| GSM6276728 |
4h overexpression, TET-promoter, IRF1, 1 |
| GSM6276729 |
4h overexpression, TET-promoter, IRF1, 2 |
| GSM6276730 |
4h overexpression, TET-promoter, IRF1, 3 |
| GSM6276731 |
4h control_sample_1 |
| GSM6276732 |
4h control_sample_2 |
| GSM6276733 |
4h control_sample_3 |
| GSM6276734 |
mutant_sample_1, IRF1 |
| GSM6276735 |
mutant_sample_2, IRF1 |
| GSM6276736 |
mutant_sample_3, IRF1 |
| GSM6276737 |
control_mutant_sample_1 |
| GSM6276738 |
control_mutant_sample_2 |
| GSM6276739 |
control_mutant_sample_3 |
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| This SubSeries is part of SuperSeries: |
| GSE207073 |
Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans |
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| Relations |
| BioProject |
PRJNA853561 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE207072_OE_2h_DE.txt.gz |
340.3 Kb |
(ftp)(http) |
TXT |
| GSE207072_OE_4h_DE.txt.gz |
339.5 Kb |
(ftp)(http) |
TXT |
| GSE207072_mutant_DE.txt.gz |
339.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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