|
| Status |
Public on Jul 01, 2022 |
| Title |
4h control_sample_1 |
| Sample type |
SRA |
| |
|
| Source name |
BWP17 parental strain
|
| Organism |
Candida albicans |
| Characteristics |
strain: BWP17 parental strain
genotype: wild-type
treatment: YPD+aTC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were mechanically opened in lysis buffer provided by the RNA extraction kit from Qiagen, with 0.5mm glasbeads during 6 rounds of 45s on a FasPrep at 6.5m speed.
Libraries were prepared with the TruSeq stranded mRNA library kit from Illumina.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
OE_4h_DE.txt
|
| Data processing |
Reads were mapped on the available C. albicans genome sequence assembly 22 (candiagenome.org) using Hisat2
DEseq2 was used for differential expression analysis assuming a fold-change of 1.5 with P-adj < 0.05 as significant
Assembly: Candida genome databse assembly 22
Supplementary files format and content: tab-delimited text files include DE values for each Sample
|
| |
|
| Submission date |
Jun 28, 2022 |
| Last update date |
Nov 09, 2022 |
| Contact name |
Lasse van Wijlick |
| E-mail(s) |
lasse.vanwijlick@pasteur.fr
|
| Organization name |
Institut Pasteur
|
| Department |
Mycology
|
| Lab |
BPF
|
| Street address |
25-28 Rue du Dr Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platform ID |
GPL19036 |
| Series (2) |
| GSE207072 |
Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans [RNA-Seq] |
| GSE207073 |
Functional Portrait of Irf1 (Orf19.217), a Regulator of Morphogenesis and Iron Homeostasis in Candida albicans |
|
| Relations |
| BioSample |
SAMN29397127 |
| SRA |
SRX15927943 |
