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| Status |
Public on Jul 10, 2022 |
| Title |
Single-nucleus and single-cell RNA-sequencing of human pancreatic islets |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptomics is frequently used to interrogate alterations in cultured human islet cells using single-cell RNA-sequencing. We introduce single-nucleus RNA-sequencing as an alternative approach for investigating human islets.
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| Overall design |
The Nuclei EZ protocol was used to obtain nuclear preparations from human islet cells. Such preparations were used to generate single-nucleus RNA-seq datasets and compared to single-cell RNA-seq output obtained from cells from the same donor.
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| Contributor(s) |
Basile G, KahramanDirice S, Kulkarni R |
| Citation(s) |
34376240 |
| |
| Submission date |
Jul 07, 2022 |
| Last update date |
Jul 10, 2022 |
| Contact name |
Hui Pan |
| E-mail(s) |
Hui.Pan@joslin.harvard.edu
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics and Biostatistics Core
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA856476 |
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