|
| Status |
Public on Jul 10, 2022 |
| Title |
single-nucleus sample 2 |
| Sample type |
SRA |
| |
|
| Source name |
Pancreatic islets
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Pancreatic islets
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Nuclei or cells were lysed in droplets using the 10X Chromium instrument.
Reverse transcription was performed in droplet and library construction was performed according to the 10X Chromium Single Cell 3' Reagent Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
SN2
sn_count_table.csv
|
| Data processing |
Raw sequencing data were demultiplexed, aligned to the human genome (GRCh38) and UMI-collapsed using CellRanger.
Assembly: GRCh38
Supplementary files format and content: CSV gene counts
|
| |
|
| Submission date |
Jul 07, 2022 |
| Last update date |
Jul 10, 2022 |
| Contact name |
Hui Pan |
| E-mail(s) |
Hui.Pan@joslin.harvard.edu
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics and Biostatistics Core
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE207632 |
Single-nucleus and single-cell RNA-sequencing of human pancreatic islets |
|
| Relations |
| BioSample |
SAMN29621161 |
| SRA |
SRX16106011 |
