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| Status |
Public on Jul 06, 2023 |
| Title |
Development of an AAV-LK03 variant with improved liver lobular biodistribution in the humanised FRG model II |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
AAV variants may differ in their tropism for human vs mouse hepatocytes or portal vs central hepatocytes. We examined the engraftment of human hepatocytes in the FRG mouse model and the transduction of hepatocytes in this model using single nuclear RNA-seq.
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| Overall design |
Single nuclear RNA-seq of human hepatocytes engrafted into FRG mouse liver, including pre- and 5 months post-engrafted, as well as AAV-treated samples
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| Contributor(s) |
Cabanes-Creus M, Navarro RG, Carlessi R, Scott S, Roca-Pinilla R, Denisenko E, Baltazar G, Liao SY, Zhu E, Wilson LW, Forrest A, Alexander IE, Tirnitz-Parker N, Lisowski L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 08, 2022 |
| Last update date |
Jul 06, 2023 |
| Contact name |
Marti Cabanes-Creus |
| E-mail(s) |
mcabanescreus@cmri.org.au
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| Organization name |
Children's Medical Research Institute
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| Street address |
214 Hawkesbury Rd
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| City |
Westmead |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL25526 |
Illumina NovaSeq 6000 (Homo sapiens; Mus musculus) |
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| Samples (10)
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| GSM6319629 |
Human hepatocytes, replicate 1, snRNAseq (dataset II) |
| GSM6319630 |
Human hepatocytes, replicate 2, snRNAseq (dataset II) |
| GSM6319631 |
Liver, 5-months post engraftment, replicate1, snRNA-seq (dataset II) |
| GSM6319632 |
Liver, 5-months post engraftment, replicate2, snRNA-seq (dataset II) |
| GSM6319633 |
Liver, 5-months post engraftment, LK03C/REDHV, replicate 1, snRNA-seq (dataset II) |
| GSM6319634 |
Liver, 5-months post engraftment, LK03C/REDHV, replicate 2, snRNA-seq (dataset II) |
| GSM6319635 |
Liver, 5-months post engraftment, LK03V/REDHC, replicate 1, snRNA-seq (dataset II) |
| GSM6319636 |
Liver, 5-months post engraftment, LK03V/REDHC, replicate 2, snRNA-seq (dataset II) |
| GSM6319637 |
Liver, 5-months post engraftment (low engraftment), replicate1, snRNA-seq (dataset II) |
| GSM6319638 |
Liver, 5-months post engraftment (low engraftment), replicate2, snRNA-seq (dataset II) |
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| Relations |
| BioProject |
PRJNA857105 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE207809_RAW.tar |
544.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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