|
| Status |
Public on Sep 09, 2011 |
| Title |
Genome-wide Analysis of Class IIa Histone Deacetylase Regulation of Hepatic Gluconeogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of Class II Histone Deacetylase (HDAC) regulation of hepatic gluconeogenesis at the gene expression level. We show that in liver, Class IIa HDACs (HDAC4, 5, and 7) are all phosphorylated and excluded from the nucleus by AMPK family kinases. In response to the fasting hormone glucagon, Class IIa HDACs rapidly translocate to the nucleus where they directly bind to the promoters of gluconeogenic enzymes such as G6Pase. In turn, HDAC4/5 mediate the acute transcriptional induction of these genes via deacetylation and activation of Foxo family transcription factors. Loss of Class IIa HDACs in the murine liver results in inhibition of FOXO target genes and lowers blood glucose, resulting in increased glycogen storage.
|
| |
|
| Overall design |
Total RNA obtained from primary hepatocytes infected with shGFP or shHDAC4 & 5 subjected to 2 or 4 hours treatment with DMSO or forskolin.
|
| |
|
| Contributor(s) |
Mihaylova MM, Shaw RJ, Yu RT |
| Citation(s) |
21565617 |
| Submission date |
Mar 19, 2010 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Ruth T Yu |
| E-mail(s) |
yu@salk.edu
|
| Phone |
858-453-4100
|
| Organization name |
Salk Institute
|
| Street address |
10010 N. Torrey Pines Rd.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6103 |
Illumina mouseRef-8 v1.1 expression beadchip |
|
| Samples (16)
|
| GSM524501 |
adenoviral GFP DMSO 2h treatment rep 1 |
| GSM524502 |
adenoviral GFP DMSO 2h treatment rep 2 |
| GSM524503 |
adenoviral GFP DMSO 4h treatment rep 1 |
| GSM524504 |
adenoviral GFP DMSO 4h treatment rep 2 |
| GSM524505 |
adenoviral GFP forskolin 2h treatment rep 1 |
| GSM524506 |
adenoviral GFP forskolin 2h treatment rep 2 |
| GSM524507 |
adenoviral GFP forskolin 4h treatment rep 1 |
| GSM524508 |
adenoviral GFP forskolin 4h treatment rep 2 |
| GSM524509 |
adenoviral shRNA HDAC4 & 5 DMSO 2h treatment rep 1 |
| GSM524510 |
adenoviral shRNA HDAC4 & 5 DMSO 2h treatment rep 2 |
| GSM524511 |
adenoviral shRNA HDAC4 & 5 DMSO 4h treatment rep 1 |
| GSM524512 |
adenoviral shRNA HDAC4 & 5 DMSO 4h treatment rep 2 |
| GSM524513 |
adenoviral shRNA HDAC4 & 5 forskolin 2h treatment rep 1 |
| GSM524514 |
adenoviral shRNA HDAC4 & 5 forskolin 2h treatment rep 2 |
| GSM524515 |
adenoviral shRNA HDAC4 & 5 forskolin 4h treatment rep 1 |
| GSM524516 |
adenoviral shRNA HDAC4 & 5 forskolin 4h treatment rep 2 |
|
| Relations |
| BioProject |
PRJNA124483 |
Less...
More...