|
| Status |
Public on Sep 09, 2011 |
| Title |
adenoviral shRNA HDAC4 & 5 forskolin 2h treatment rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
primary hepatocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 6-8 wks
cell type: hepatocytes
treatment: adenoviral shRNA HDAC4 & 5 forskolin
time: 2h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalized using cubic spline in Illumina GenomeStudio and also using the VAMPIRE Bayesian model with a 2-sided Bonferroni-corrected threshold of 0.05.
|
| |
|
| Submission date |
Mar 19, 2010 |
| Last update date |
Sep 09, 2011 |
| Contact name |
Ruth T Yu |
| E-mail(s) |
yu@salk.edu
|
| Phone |
858-453-4100
|
| Organization name |
Salk Institute
|
| Street address |
10010 N. Torrey Pines Rd.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE20979 |
Genome-wide Analysis of Class IIa Histone Deacetylase Regulation of Hepatic Gluconeogenesis |
|
