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| Status |
Public on Jun 01, 2011 |
| Title |
The CRE1 carbon catabolite repressor of the fungus Trichoderma reesei: a master regulator of carbon assimilation |
| Organism |
Trichoderma reesei |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
The identification and characterization of the transcriptional regulatory networks governing the physiological behaviour and adaptation of microbial cells is a key step in understanding their behaviour. One such wide-domain regulatory circuit, essential to all cells, is carbon catabolite repression (CCR): it allows the cell to prefer some carbon sources, whose assimilation is of high nutritional value, over less profitable ones. This system has been investigated in bacteria, yeast and filamentous fungi. In the latter, the C2H2 zinc finger protein has been shown to act as the central transcriptional repressor in this process. Here, we deciphered the CRE1 regulon by profiling transcription in a wild-type and delta-cre1 mutant strains on glucose in the model cellulose and hemicellulose-degrading fungus Trichoderma reesei (anamorph of Hypocrea jecorina) at constant growth rates known to per se repress and derepress CCR-affected genes.
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| Overall design |
Two biological pool by condition in dye switch. For the two biological replicates on each four experiments we apply on the pretreated results the linear modeling approach implemented by lmFit and the empirical Bayes statistics implemented by eBayes from the limma R package (Smyth 2004). We select the list of statistically regulated genes using a 5% significance threshold.
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| Contributor(s) |
Le Crom S, Kubicek C, Portnoy T, Linke R, Margeot A, Seiboth B |
| Citation(s) |
21619626 |
| |
| Submission date |
Mar 25, 2010 |
| Last update date |
Jul 07, 2014 |
| Contact name |
Stéphane LE CROM |
| Organization name |
École normale supérieure
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| Department |
Biology Institute - IBENS
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| Lab |
Genomic platform
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| Street address |
46 rue d'Ulm
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| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
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| Platforms (1) |
| GPL9076 |
ENS SGDB Trichoderma reesei Tr20_Tiling140_1 240k oligo array |
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| Samples (8)
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| GSM526857 |
QM9414 strain deleted for Cre1 versus QM9411 strain under carbon catabolite repression |
| GSM526858 |
QM9414 strain deleted for Cre1 versus QM9411 strain under carbon catabolite repression dye switch |
| GSM526859 |
QM9414 strain deleted for Cre1 versus QM9411 strain under carbon catabolite derepression |
| GSM526860 |
QM9414 strain deleted for Cre1 versus QM9411 strain under carbon catabolite derepression dye switch |
| GSM526861 |
QM9414 strain under carbon catabolite repression versus under carbon catabolite derepression |
| GSM526862 |
QM9414 strain under carbon catabolite repression versus under carbon catabolite derepression dye switch |
| GSM526863 |
QM9414 strain deleted for Cre1 under carbon catabolite repression versus under carbon catabolite derepression |
| GSM526864 |
QM9414 strain deleted for Cre1 under carbon catabolite repression versus under carbon catabolite derepression dye switch |
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| Relations |
| BioProject |
PRJNA126739 |
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