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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 29, 2011 |
| Title |
Accelerated leukemogenesis by truncated CBFb-SMMHC defective in high-affinity binding with RUNX1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dominant RUNX1 inhibition has been proposed as a common pathway for CBF-leukemia. CBFb-SMMHC, a fusion protein in human acute myeloid leukemia (AML), dominantly inhibits RUNX1 largely through its RUNX1 high-affinity binding domain (HABD). We generated knock-in mice expressing CBFb-SMMHC with a HABD deletion, CBFb-SMMHCd179-221. These mice developed leukemia highly efficiently, even though hematopoietic defects associated with Runx1-inhibition were partially rescued.
To identify changes in gene expression with the deletion of the HABD, we compared the gene expression profile in leukemia samples from mice expressing CBFb-SMMHCd179-221 with those from mice expressing full length CBFb-SMMHC.
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| Overall design |
Spleen cells were isolated from leukemic knock-in mice with full length CBFb-SMMHC at 2 months after ENU treatment and 2 leukemic CBFb-SMMHCd179-221 expressing chimeric mice at 3 weeks after birth. For each genotype, we performed two independent experiments with 4 Affymetrix GeneChip 430 chips.
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| Contributor(s) |
Kamikubo Y, Zhao L, Wunderlich M, Corpora T, Hyde RK, Paul T, Kundu M, Garrett-Beal L, Compton S, Huang G, Wolff L, Ito Y, Bushweller J, Mulloy JC, Liu PP |
| Citation(s) |
20478528 |
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| Submission date |
Mar 31, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Christopher Pan |
| E-mail(s) |
cpan@mail.nih.gov
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| Organization name |
NIH
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| Department |
NHGRI
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| Street address |
Rm 5228, Bldg 50, South Dr.
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| GSM529593 |
CBFb-SMMHC animal 2, replicate 3 |
| GSM529594 |
CBFb-SMMHCd179-221 animal 1, replicate 1 |
| GSM529595 |
CBFb-SMMHCd179-221 animal 2, replicate 1 |
| GSM529596 |
CBFb-SMMHCd179-221 animal 2, replicate 2 |
| GSM529597 |
CBFb-SMMHCd179-221 animal 2, replicate 3 |
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| Relations |
| BioProject |
PRJNA126601 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21155_RAW.tar |
28.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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