|
| Status |
Public on Apr 24, 2023 |
| Title |
Off-target effect of engineered AsCas12f variants |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Cells were transfected with plasmids containing AsCas12f variants and sgRNAs for gene-editing. Off-target effect was determined using GUIDE-seq on an Illumina Nextseq platform.
|
| |
|
| Overall design |
GUIDE-seq
|
| Web link |
https://www.nature.com/articles/s41589-023-01380-9
|
| |
|
| Contributor(s) |
Wu T, Lyu R, Tang W |
| Citation(s) |
37400536 |
| Submission date |
Aug 18, 2022 |
| Last update date |
Jul 24, 2023 |
| Contact name |
Tong Wu |
| E-mail(s) |
tongwu@uchicago.edu
|
| Organization name |
University of Chicago
|
| Street address |
929 E 57th St. GCIS E335
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL30173 |
NextSeq 2000 (Homo sapiens) |
|
| Samples (34)
|
|
| Relations |
| BioProject |
PRJNA871035 |
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