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Status |
Public on Apr 24, 2023 |
Title |
AsCas12f-WT-HEXA-rep2 |
Sample type |
SRA |
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Source name |
embryonic kidney
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Organism |
Homo sapiens |
Characteristics |
tissue: embryonic kidney cell line: HEK293T cell type: Kidney epithelial genotype: Wild type treatment: Nucleofection ascas12f version: WT guide rna_target: HEXA
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Growth protocol |
HEK293T cells were purchased from (ATCC, CRL11268) were cultured in DMEM (Gibco 11995) supplemented with 10% (v/v) fetal bovine serum (Gibco), 1% penicillin and streptomycin (Gibco, 15140).
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Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using Purelink Genomic DNA Mini Kit (Thermo Fisher, K1820) 1 µg gDNA was applied to fragmentation, end-repair, A-tailing, adapter ligation, and dsODN-specific amplification, following the published GUIDE-seq protocol.
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Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NextSeq 500 |
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Description |
GUIDE-seq
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Data processing |
GUIDE-seq was analysed using an open-source guideseq software: https://github.com/aryeelab/guideseq Assembly: hg19 Supplementary files format and content: read density that can be used for visualization on genome browsers. Library strategy: GUIDE-seq
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Submission date |
Aug 18, 2022 |
Last update date |
Apr 24, 2023 |
Contact name |
Tong Wu |
E-mail(s) |
tongwu@uchicago.edu
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Organization name |
University of Chicago
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Street address |
929 E 57th St. GCIS E335
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City |
Chicago |
State/province |
Illinois |
ZIP/Postal code |
60637 |
Country |
USA |
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Platform ID |
GPL18573 |
Series (1) |
GSE211600 |
Off-target effect of engineered AsCas12f variants |
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Relations |
BioSample |
SAMN30399927 |
SRA |
SRX17145576 |
Supplementary file |
Size |
Download |
File type/resource |
GSM6481330_GUIDE-seq.TW10_dP_WT-2.bedgraph.gz |
9.8 Mb |
(ftp)(http) |
BEDGRAPH |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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