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| Status |
Public on Apr 27, 2023 |
| Title |
Uridylation is a genomic feature of encapsidated GFLV RNAs [Purified virions] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using 3' RACE-seq method, we measured uridylation frequencies of GFLV RNAs (K30 and B844 isolates) for total RNA of C. quinoa infected plants or for purified RNA from GFLV virions.
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| Overall design |
3’ RACE-seq method was used to measure poly(A) tail sizes and analyze uridylation levels. This protocol is based on the ligation of a primer at the 3’ end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.
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| Contributor(s) |
Joly A, Zuber H, Gagliardi D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 30, 2022 |
| Last update date |
Apr 27, 2023 |
| Contact name |
Dominique Gagliardi |
| E-mail(s) |
dominique.gagliardi@ibmp-cnrs.unistra.fr
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| Organization name |
CNRS
|
| Department |
IBMP
|
| Street address |
12, rue du General Zimmer
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| City |
Strasbourg |
| ZIP/Postal code |
67084 |
| Country |
France |
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| Platforms (1) |
| GPL17970 |
Illumina MiSeq (Arabidopsis thaliana) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE212358 |
A global survey of phytoviral RNA 3' uridylation identifies extreme variations and virus-specific patterns |
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| Relations |
| BioProject |
PRJNA875116 |
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