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Status |
Public on Apr 27, 2023 |
Title |
Uridylation is a genomic feature of encapsidated GFLV RNAs [Purified virions] |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Using 3' RACE-seq method, we measured uridylation frequencies of GFLV RNAs (K30 and B844 isolates) for total RNA of C. quinoa infected plants or for purified RNA from GFLV virions.
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Overall design |
3’ RACE-seq method was used to measure poly(A) tail sizes and analyze uridylation levels. This protocol is based on the ligation of a primer at the 3’ end of RNA, and the subsequent targeted amplification by PCR of amplicons suitable for Illumina sequencing.
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Contributor(s) |
Joly A, Zuber H, Gagliardi D |
Citation missing |
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Submission date |
Aug 30, 2022 |
Last update date |
Apr 27, 2023 |
Contact name |
Dominique Gagliardi |
E-mail(s) |
dominique.gagliardi@ibmp-cnrs.unistra.fr
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Organization name |
CNRS
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Department |
IBMP
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Street address |
12, rue du General Zimmer
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City |
Strasbourg |
ZIP/Postal code |
67084 |
Country |
France |
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Platforms (1) |
GPL17970 |
Illumina MiSeq (Arabidopsis thaliana) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE212358 |
A global survey of phytoviral RNA 3' uridylation identifies extreme variations and virus-specific patterns |
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Relations |
BioProject |
PRJNA875116 |