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| Status |
Public on Nov 05, 2022 |
| Title |
Genome-wide investigation of transcriptional regulation in malaria sporozoite [RNA-Seq] |
| Organism |
Plasmodium berghei |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sporozoite is the stage in which malaria parasites initially infect the vertebrate host. Elucidation of gene regulation in this stage will promote the investigation of mechanisms of liver infection by this parasite and contribute to development of strategies for preventing the malaria transmission. AP2-Sp is a transcription factor essential for formation of sporozoites or sporogony, which take place in oocysts on the midgut of infected mosquitoes. To understand the role of this transcription factor in the transcriptional regulatory system of this stage we performed ChIP-seq analysis using whole mosquito midguts containing late oocysts as start materials and explore its target genes genome-widely. Target genes were composed of 640 genes, which encompassed various functional categories and were contained genes involved in distinct processes parasites pass through in this stage, from sporogony to development into the liver stage. Furthermore, RNA-seq analysis showed that these genes constituted majority of the genes highly expressed in in this stage. These results suggested that this TF determines basal pattern of gene expression of this stage by targeting a broad range of genes directly.
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| Overall design |
Plasmodium berghei transcriptomes were analyzed in oocyst/oocyst sporozoites and salivary gland sporozoites.
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| Contributor(s) |
Yuda M, Kaneko I, Iwanaga S, Nishi T, Murata Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 06, 2022 |
| Last update date |
Nov 05, 2022 |
| Contact name |
masao yuda |
| E-mail(s) |
m-yuda@doc.medic.mie-u.ac.jp
|
| Organization name |
mie university
|
| Street address |
edobashi 2-174
|
| City |
tsu |
| State/province |
mie |
| ZIP/Postal code |
514-0001 |
| Country |
Japan |
| |
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| Platforms (2) |
| GPL23133 |
Illumina NextSeq 500 (Plasmodium berghei) |
| GPL32636 |
DNBSEQ-G400 (Plasmodium berghei) |
|
| Samples (8)
|
| GSM6544426 |
oocysts/oocyst sporozoites_RNA-seq_experiment-1 |
| GSM6544427 |
oocysts/oocyst sporozoites_RNA-seq_experiment-2 |
| GSM6544428 |
oocysts/oocyst sporozoites_RNA-seq_experiment-3 |
| GSM6544429 |
salivary gland sporozoitess_RNA-seq_experiment-1 |
| GSM6544430 |
salivary gland sporozoitess_RNA-seq_experiment-2 |
| GSM6544431 |
salivary gland sporozoitess_RNA-seq_experiment-3 |
| GSM6544432 |
AP2-Sp_cismut_clone1_RNA-seq |
| GSM6544433 |
AP2-Sp_cismut_clone2_RNA-seq |
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| This SubSeries is part of SuperSeries: |
| GSE212754 |
Genome-wide investigation of transcriptional regulation in malaria sporozoite |
|
| Relations |
| BioProject |
PRJNA877203 |
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