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| Status |
Public on Aug 20, 2010 |
| Title |
The role of microRNAs in hepatic thyroid hormone signalling |
| Platform organisms |
Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Other
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| Summary |
MicroRNAs (miRNAs) are extensively in diverse biological processes. However, very little is known about the role of miRNAs in mediating thyroid hormones (TH) action. Maintenance of appropriate TH levels is known to be critically important for development, differentiation and maintenance of metabolic balance in mammals. We induced transient hypothyroidism in juvenile mice by short term exposure to methimazole and perchlorate from post natal day (PND) 12 to 15. The expression of miRNAs in the liver was analyzed with the Taqman Low Density Array (containing up to 600 rodent microRNAs). We found the expression of 40 miRNAs was significantly altered in the liver of hypothyroid mice compared to euthyroid controls. Among the miRNAs, miR-1/206/133 exhibited a massive increase in expression (50 – 500 folds). To further explore TH effect on miR-1/206/133, we treated mouse hepatocyte AML 12 cells with 10 nm T3 for 1 hr and 24 hrs. TH treatment induced the down-regulation of miR-1/206/133b at both time points, reaching statistical significance at 24 hrs. To identify the genes targeted by these miRNAs, DNA microarrays were used to examine hepatic mRNA levels in the hypothyroid mouse model alongside controls. We found transcripts from 92 known genes were significantly altered in hypothyroid mice. Web-based target predication softwares (TargetScan and Microcosm) identified 14 of these transcripts as targets of miR-1/106/133. The vast majority of these mRNA targets were significantly down-regulated in hypothyroid mice, corresponding well with the up-regulation of miR-1/206/133 in hypothyroid mouse liver. The results suggest that TH regulation of these genes may occur secondarily via the reduced repression consequent to TH-induced down-regulation of miR-1/206/133. These studies provide novel insight into the role of miRNAs in mediating TH regulation of gene expression.
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| Overall design |
Agilent two-color microarray were used to perform gene expression profile in euthyroid and hypothyroid male mouse livers, 5 biological replicates. TLDA (Taqman microRNA Array) was used to explore the miRNA expression profile in euthyroid and hypothyroid male mouse livers, 4 biological replicates.
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| Contributor(s) |
Dong H, Paquette M, Williams A, Zoeller RT, Wade M, Yauk C |
| Citation(s) |
20808432 |
| Submission date |
Apr 09, 2010 |
| Last update date |
May 10, 2018 |
| Contact name |
hongyan dong |
| E-mail(s) |
hongyan_dong@hc-sc.gc.ca
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| Organization name |
health canada
|
| Street address |
50 colombine Dr.
|
| City |
ottawa |
| ZIP/Postal code |
k1y0l9 |
| Country |
Canada |
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| Platforms (2) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
| GPL10331 |
TaqMan® Rodent miRNA A+B Cards Set v2.0 |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA126581 |
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