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Series GSE212831 Query DataSets for GSE212831
Status Public on Sep 07, 2022
Title Cell-type identity of the avian utricle
Organism Gallus gallus
Experiment type Expression profiling by high throughput sequencing
Summary The avian utricle, a vestibular organ of the inner ear, displays turnover of sensory hair cells throughout life. This is in sharp contrast to the mammalian utricle, which shows limited regenerative capacity. Here, we use single-cell RNA-sequencing to identify distinct marker genes for the different sensory hair cell subtypes of the chicken utricle, which we validated in situ . We provide markers for spatially distinct supporting cell populations, and identified two transitional cell populations of dedifferentiating supporting cells and developing hair cells. Trajectory reconstruction resulted in an inventory of gene expression dynamics of natural hair cell generation in the avian utricle.
 
Overall design Utricles were dissected, single cells were deposited into individual wells of 96-well plates using flow cytometry and processed for single-cell RNA-sequencing
 
Contributor(s) Scheibinger M, Heller S
Citation(s) 36170825
Submission date Sep 07, 2022
Last update date Nov 02, 2022
Contact name Daniel C Ellwanger
Organization name Amgen Inc
Department Genome Analysis Unit
Street address 1120 Veterans Blvd
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platforms (1)
GPL19787 Illumina NextSeq 500 (Gallus gallus)
Samples (300)
GSM6555893 Gallus gallus utricle, cell-001
GSM6555894 Gallus gallus utricle, cell-002
GSM6555895 Gallus gallus utricle, cell-003
Relations
BioProject PRJNA877608

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE212831_RAW.tar 17.1 Mb (http)(custom) TAR (of CSV)
GSE212831_allsamples_raw_counts.csv.gz 1.8 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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