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| Status |
Public on Sep 25, 2023 |
| Title |
The environmentally-regulated interplay between three-dimensional chromatin organisation and transcription of the Escherichia coli proVWX operon |
| Organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Other
|
| Summary |
Nucleoid associated proteins maintain the architecture of bacterial chromosomes and regulate gene expression. Thus, their role as transcription factors may involve three-dimensional chromosome re-modelling. Here, we provide the first in vivo evidence supporting this hypothesis. Using RT-qPCR and 3C-qPCR, we show that activation of the H-NS-regulated, osmosensitive proVWX operon of Escherichia coli in response to a hyperosmotic shock involves the destabilization of H-NS-mediated bridges anchored between the proVWX downstream and upstream regulatory elements (DRE and URE), and between the DRE and ygaY that lies immediately downstream of proVWX. The re-establishment of these bridges in cells adapted to hyperosmolarity represses the operon. While the DRE—H-NS—URE bridge is disrupted by hyperosmolarity, the DRE—H-NS—ygaY bridge weakens and requires active transcription to be dismantled. H-NS and H-NS-like proteins are wide-spread amongst bacteria, suggesting that structural regulation of expression may be a typical feature of transcriptional control in bacteria.
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| Overall design |
Chromosome conformation capture experiments to test how various cell fixation techniques, and the enrichment of DNA-protein complexes impact the signal-to-noise ratio in chromosome contact maps.
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| Contributor(s) |
Rashid FM, Hofmann A, Heermann DW, Crémazy FG, Dame RT, Grainger DC, Forrest D |
| Citation(s) |
37978176 |
| |
| Submission date |
Sep 30, 2022 |
| Last update date |
Dec 08, 2023 |
| Contact name |
Remus Dame |
| E-mail(s) |
rtdame@chem.leidenuniv.nl
|
| Phone |
+31715275605
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| Organization name |
Leiden University
|
| Department |
Leiden Institute of Chemistry
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| Lab |
Macromolecular biochemistry
|
| Street address |
Einsteinweg 55
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| City |
Leiden |
| State/province |
Zuid-Holland |
| ZIP/Postal code |
2333CC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL21117 |
Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA885782 |
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