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| Status |
Public on Apr 12, 2023 |
| Title |
IL15 Deficiency in Rats |
| Platform organism |
Rattus norvegicus |
| Sample organism |
Rattus |
| Experiment type |
Expression profiling by array
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| Summary |
NK cells are a major immune cell in the uterus during pregnancy. IL15 is required for the maturation of these cells, therefore a mutation in the IL15 gene results in a lack of NK cells. In this study, implantation sites were collected from gestational day 9.5 pregnant IL15 deficient or WT Sprague-Dawley Holtzman rats. RNA was collected, and a Clariom S microarray was performed to elucidate differences in gene expression at the developing placenta and uterus between mutants and WT dams.
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| Overall design |
WT dams and IL15Δ/Δ dams were mated with a WT male. On GD 9.5 implantation sites (containing developing placenta and embryo) were collected and RNA extracted using a TRIzol-RNeasy column hybrid protocol including on-column DNase I digestion, and purified. Each sample represents RNA pooled from 3 implantation sites a single dam. Normalization and output file generation was performed with Affymetrix Gene Expression Console (Thermo) using the Robust Multi-Chip Analysis (RMA) algorithm [RMA-Gene-full].
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| Contributor(s) |
Baines K, Renaud SJ |
| Citation(s) |
37152295 |
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| Submission date |
Oct 24, 2022 |
| Last update date |
Jul 12, 2023 |
| Contact name |
Stephen J Renaud |
| Organization name |
University of Western Ontario
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| Street address |
1151 Richmond St, MSB428
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| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 5C1 |
| Country |
Canada |
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| Platforms (1) |
| GPL23040 |
[Clariom_S_Rat] Affymetrix Clariom S Assay, Rat (Includes Pico Assay) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA893659 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216395_RAW.tar |
5.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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