|
| Status |
Public on Apr 12, 2023 |
| Title |
WT, dam 1 |
| Sample type |
RNA |
| |
|
| Source name |
Gestational day 9.5 Imp. Site from WT
|
| Organism |
Rattus |
| Characteristics |
tissue: Placenta
developmental stage: Gestational day 9.5
genotype: WT
|
| Treatment protocol |
Implantation sites were isolated on gestational day 9.5, frozen in liquid nitrogen, and maintained at -80C until RNA extraction.
|
| Growth protocol |
Dams were aged 6-8 weeks before mating with a WT male. Implantation sites were isolated on gestaional day 9.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen placental tissue was homogenized in TRIzol, the aqueous phase removed following mixing in chloroform, mixed with 70% ethanol, and then placed onto an Rneasy column (Qiagen). Following on-column Dnase I digestion, RNA was purified. RNA from two placentas per dam was pooled.
|
| Label |
biotin
|
| Label protocol |
Sense strand cDNA was generated, fragmented, and labelled using the GeneChip WT Plus Reagent kit (ThermoFisher) as per directions.
|
| |
|
| Hybridization protocol |
Samples were hybridized to the Clariom S GeneChip microarray (ThermoFisher) as per directions from the GeneChip WT Plus Reagent kit (ThermoFisher). Overnight hybridization to the Clariom S GeneChip took place at 45C for ~16 h.
|
| Scan protocol |
After washing and staining as per directions for the GeneChip WT Plus Reagent kit (ThermoFisher), images were captured using the GeneChip Scanner 3000 and raw data converted to .CEL files using the Affymetrx GCOS software (ThermoFisher).
|
| Description |
Gene expression data from placentas isolated from dams fed a normal diet
|
| Data processing |
The CEL file was uploaded to Transcriptome Analysis Console Sotware 4.0 (ThermoFisher) to analyze expression patterns of genes, pathways, and network interactions.
|
| |
|
| Submission date |
Oct 24, 2022 |
| Last update date |
Apr 14, 2023 |
| Contact name |
Stephen J Renaud |
| Organization name |
University of Western Ontario
|
| Street address |
1151 Richmond St, MSB428
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 5C1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL23040 |
| Series (1) |
|
