|
| Status |
Public on Nov 07, 2022 |
| Title |
Targeted modulation of chicken genes in vitro using CRISPRa and CRISPRi toolkit |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the effectiveness of CRISPR based gene modulation, IRF7 was repressed in dCas9-KRAB DF1 cell line or activated in dCas9-VPR DF1 cell line followed by whole transcriptome analysis
|
| |
|
| Overall design |
a. Differntiall gene expression analysis between mock (non-targeting) and IRF7 targeting gRNAs transfected dCas9-KRAB DF1 cells for assessing the endogenous targeted gene knockdown
b. Differntiall gene expression analysis between mock (non-targeting) and IRF7 targeting gRNAs transfected dCas9-VPR DF1 cells for assessing the endogenous targeted gene activation
|
| |
|
| Contributor(s) |
Chapman B, Han JH, Lee HJ, Rudd I, Kim TH |
| Citation(s) |
37107664 |
| |
| Submission date |
Nov 04, 2022 |
| Last update date |
May 12, 2023 |
| Contact name |
Tae Hyun Kim |
| E-mail(s) |
tpk5258@psu.edu
|
| Organization name |
Penn State University
|
| Street address |
317 AVBS
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
|
| Samples (8)
|
| GSM6710880 |
dCas9-KRAB DF1, CRISPRi IRF7 targted knockdown_A |
| GSM6710881 |
dCas9-KRAB DF1, CRISPRi IRF7 targted knockdown_B |
| GSM6710882 |
dCas9-KRAB DF1, Mock control_A |
|
| Relations |
| BioProject |
PRJNA898374 |
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