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| Status |
Public on Mar 29, 2023 |
| Title |
RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding [scRNA-seq_stopping_Scipio] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Progress in sample preparation for scRNA-seq is reported based on RevGel-seq, a reversible-hydrogel technology. Barcode bead-cell tandems stabilized by a chemical linker are dispersed in the hydrogel in the liquid state. Upon gelation the tandems are immobilized, cell lysis is triggered by detergent diffusion, and RNA molecules are captured on the adjacent barcode beads. After reverse transcription and preparation for cDNA sequencing, bioinformatic analysis reveals performance quality comparable to microfluidic-based technologies.
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| Overall design |
Benchmarking experiments to assess the performances obtained using RevGel-seq (scRNA preparation).
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| Contributor(s) |
Fernandez N, Poncin R, Godet U, Eckert H, Saffarien A, Lipin S, Ulveling D, Fourne Y, Edelstein S |
| Citation(s) |
36964177 |
| Submission date |
Nov 17, 2022 |
| Last update date |
Mar 30, 2023 |
| Contact name |
Stuart EDELSTEIN |
| Organization name |
Scipio bioscience
|
| Street address |
29 rue du Faubourg Saint Jacques, Pépinière Paris Santé Cochin
|
| City |
Paris |
| State/province |
Île-de-France |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (1) |
| GPL25526 |
Illumina NovaSeq 6000 (Homo sapiens; Mus musculus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE218213 |
RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding |
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| Relations |
| BioProject |
PRJNA902749 |
