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| Status |
Public on Mar 01, 2023 |
| Title |
Verson’s glands contribute to molting and antimicrobial response in the Fall armyworm |
| Organism |
Spodoptera frugiperda |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Verson’s glands are segmental pairs of dermal glands attached to the epidermis in lepidopteran larvae. They produce macromolecules during intermolt period and empty them during each molt. Morphological, histochemical, developmental, and protein analysis studies have been conducted to determine the functions of Verson’s glands. However, the exact role of Verson’s glands remains unclear. In our previous study, a strain of transgenic fall armyworm, Spdoptera frugiperda expressing green fluorescence protein (GFP) and Systemic RNA interference defective protein 1 (SID-1) from Caenorhabditis elegans was established to improve RNA interference (RNAi) efficiency. Unexpectedly, we found that GFP fluorescence was significantly brighter in Verson’s glands than in other tissues. Also, RNAi efficiency improved more in Verson’s glands than in other tissues. We took advantage of improved RNAi efficiency to explore the function of Verson’s glands. RNA-seq analysis revealed that genes highly expressed in Verson’s glands code for cuticular proteins, molting fluid proteins, hemolymph proteins, and antimicrobial peptides. Injection of dsRNA targeting essential genes interfered with Verson’s glands growth. These studies revealed that Verson’s glands contribute to hemolymph, cuticle, molting fluid, and immune response during molting. These studies also provide useful tools for future research in identifying the physiological role of Verson’s glands in lepidopteran insects.
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| Overall design |
Comparative gene expression analysis of RNA-seq data for Spodoptera frugiperda Verson's glands and other tissues (fat body, midgut, epidermis from bioproject PRJNA646922)
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| Web link |
https://www.frontiersin.org/articles/10.3389/finsc.2023.1124278/full
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| Contributor(s) |
Koo J, Chen X, Palli SR |
| Citation(s) |
38469461 |
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Koo et. al., Front. Insect Sci., 10 February 2023
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| Submission date |
Dec 14, 2022 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Jin mo Koo |
| E-mail(s) |
jko256@uky.edu
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| Organization name |
University of Kentucky
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| Street address |
100 South Limestone Street
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| City |
Lexington |
| State/province |
Kentucky |
| ZIP/Postal code |
40546 |
| Country |
USA |
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| Platforms (1) |
| GPL32951 |
BGISEQ-500 (Spodoptera frugiperda) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA912203 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220983_RAW.tar |
2.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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