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| Status |
Public on Jan 19, 2023 |
| Title |
LncRNA CCRR Attenuates Inflammatory Response after Myocardial Infarction by Inhibiting TLR Signaling Pathway |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Inflammatory response plays a bidirectional regulatory role in myocardial infarction (MI). TLR signaling pathway plays an important role in the development of inflammation. Additionally, we discovered that CCRR is an anti-arrhythmic lncRNA in a prior work. Here we show, it is decreased in mice with 3-day MI followed by a sharp increase of pro-inflammatory cytokines and activation of the TLR pathway. Overexpression of CCRR could effectively inhibit the inflammatory response, reduce the infarct area, and improve cardiac function. And also reduced hypoxia-induced inflammation in cardiomyocytes and macrophages induced by IFN and LPS. It produced a similar protective effect in cardiomyocytes co-cultured with macrophages. Through microarray analysis, RNA-binding protein immunoprecipitation analysis, and other related experiments verification, TLR2 and TLR4 may be as potential targets of CCRR after MI. Our findings provide the evidence that lncRNA CCRR plays a key cardioprotective role against MI injury by targeting the TLR signaling pathway.
nflammatory response plays a bidirectional regulatory role in myocardial infarction (MI). TLR signaling pathway plays an important role in the development of inflammation. Additionally, we discovered that CCRR is an anti-arrhythmic lncRNA in a prior work. Here we show, it is decreased in mice with 3-day MI followed by a sharp increase of pro-inflammatory cytokines and activation of the TLR pathway. Overexpression of CCRR could effectively inhibit the inflammatory response, reduce the infarct area, and improve cardiac function. And also reduced hypoxia-induced inflammation in cardiomyocytes and macrophages induced by IFN and LPS. It produced a similar protective effect in cardiomyocytes co-cultured with macrophages. Through microarray analysis, RNA-binding protein immunoprecipitation analysis, and other related experiments verification, TLR2 and TLR4 may be as potential targets of CCRR after MI. Our findings provide the evidence that lncRNA CCRR plays a key cardioprotective role against MI injury by targeting the TLR signaling pathway.
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| Overall design |
The expression of heart mRNA was detected in 12 samples in 4 subgroups of WT, TG-CCRR,WT+MI,TG-CCRR+MI, with 3 samples in each group.
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| Contributor(s) |
Sun L, Wang S |
| Citation missing |
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| Submission date |
Jan 18, 2023 |
| Last update date |
Jan 23, 2023 |
| Contact name |
Lihua Sun |
| E-mail(s) |
wangsjsjts@outlook.com
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| Organization name |
Harbin Medical University
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| Street address |
157 Baojian Road
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| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150081 |
| Country |
China |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA925218 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223208_RAW.tar |
161.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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