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| Status |
Public on Jan 25, 2023 |
| Title |
The novel chloroplast glucose transporter pGlcT2 affects adaptation to extended light periods |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intracellular sugar compartmentation is critically involved in plant development and acclimation to challenging environmental conditions. Sugar transport proteins are present in the plasma membrane and in membranes of organelles such as vacuoles, the Golgi apparatus and plastids. Here we report on a novel transporter of the Monosaccharide Transporter Family (MSF), that is the closest phylogenetic homolog to the chloroplast-localized glucose transporter pGlcT and that we therefore term plastidic glucose transporter 2 (pGlcT2). Its gene complemented glucose uptake deficiency of an Escherichia coli ptsG/manXYZ mutant strain and biochemical characterization revealed that this transporter specifically facilitates glucose transport, whereas other sugars do not serve as substrates. pGlcT2-GFP fusions located to the chloroplast envelope, and promoter activity analyses indicated that pGlcT2 is mainly produced in seedlings and in the rosette center of mature Arabidopsis plants. In the absence of additional sugars, pGlcT2-overexpression lines exhibited impaired germination. In conjunction with molecular and metabolic data we propose that pGlcT2 acts as a glucose importer that can limit cytosolic glucose availability in developing pGlcT2-overexpressing seedlings. Overexpression, as well as deletion of pGlcT2 resulted in impaired growth efficiency under long day and especially under continuous light conditions, suggesting that pGlcT2 contributes to a release of glucose derived from starch mobilization late in the light phase. These data thus indicate that the facilitator pGlcT2 changes its direction of glucose transport during plant development and suggest that the activity of pGlcT2 must be controlled spatially and temporarily, in order to prevent developmental defects during adaptation to periods of extended light.
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| Overall design |
Comparative gene expression analysis of Arabidopsis thaliana lines overexpressing or lacking the pGLCT2 (At1g05030) gene under permanent light conditions
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| Web link |
https://doi.org/10.1016/j.jbc.2023.104741
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| Contributor(s) |
Marzieh V, Isabel K, Benjamin P, H Ekkehard N |
| Citation(s) |
37088133 |
| Submission date |
Jan 20, 2023 |
| Last update date |
Apr 26, 2023 |
| Contact name |
Benjamin Pommerrenig |
| E-mail(s) |
pommerre@bio.uni-kl.de
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| Organization name |
University of Kaiserslautern
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| Department |
Biology
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| Lab |
Ekkehard Neuhaus
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| Street address |
Paul-Ehrlich-Str. 22
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| City |
Kaiserslautern |
| ZIP/Postal code |
67663 |
| Country |
Germany |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (12)
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| GSM6945711 |
leaf tissue, wild-type, permanent light, biol rep 1 |
| GSM6945712 |
leaf tissue, wild-type, permanent light, biol rep 2 |
| GSM6945713 |
leaf tissue, wild-type, permanent light, biol rep 3 |
| GSM6945714 |
leaf tissue, wild-type, permanent light, biol rep 4 |
| GSM6945715 |
leaf tissue, pGLCT2 overexpressor, permanent light, biol rep 1 |
| GSM6945716 |
leaf tissue, pGLCT2 overexpressor, permanent light, biol rep 2 |
| GSM6945717 |
leaf tissue, pGLCT2 overexpressor, permanent light, biol rep 3 |
| GSM6945718 |
leaf tissue, pGLCT2 overexpressor, permanent light, biol rep 4 |
| GSM6945719 |
leaf tissue, pGLCT2 knock out, permanent light, biol rep 1 |
| GSM6945720 |
leaf tissue, pGLCT2 knock out, permanent light, biol rep 2 |
| GSM6945721 |
leaf tissue, pGLCT2 knock out, permanent light, biol rep 3 |
| GSM6945722 |
leaf tissue, pGLCT2 knock out, permanent light, biol rep 4 |
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| Relations |
| BioProject |
PRJNA925827 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223330_Valifard_et_al-Count-fpkm.xlsx |
8.7 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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