|
| Status |
Public on Jun 07, 2023 |
| Title |
Gene expression of Dcir-/- osteoclasts induced by M-CSF and RANKL |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: DCIR is an inhibitory type of C-type lectin receptor that regulates osteoclast (OC) formation and functions. This study aimed to find the genes of OCs that could be regulated through DCIR.
Method: Bone marrow cells from WT and Dcir-/- mice were cultured in M-CSF for 2 days and the the cells were further cultured in M-CSF and RANKL. RNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq.
Results: We detected xxx gense that were higher expression in Dcir-/- OCs, compared with WT OCs, with a fold change ≥1.5 and p value <0.05.
Conclusion: Our data showed that Dcir deficiency in OCs changed the gene expression pattern, compared to WT OCs, which are induced by M-CSF and RANKL.
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| |
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| Overall design |
mRNA profiles of WT and Dcir-/- osteoclasts for 2 days culture aftre RANKL stimulation
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| |
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| Contributor(s) |
Shimizu K |
| Citation(s) |
37266426 |
| |
| Submission date |
Jan 21, 2023 |
| Last update date |
Jun 07, 2023 |
| Contact name |
Tomonori Kaifu |
| E-mail(s) |
kaifu@tohoku-mpu.ac.jp
|
| Phone |
+817270212
|
| Organization name |
Tohoku Medicine and Pharmaceutical University
|
| Department |
Faculty of Medicine
|
| Lab |
Division of Immunology
|
| Street address |
1-15-1 Fukumuro Miyagino ku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
9838536 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA926132 |
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