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Series GSE223429 Query DataSets for GSE223429
Status Public on Jun 07, 2023
Title Gene expression of Dcir-/- osteoclasts induced by M-CSF and RANKL
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: DCIR is an inhibitory type of C-type lectin receptor that regulates osteoclast (OC) formation and functions. This study aimed to find the genes of OCs that could be regulated through DCIR.
Method: Bone marrow cells from WT and Dcir-/- mice were cultured in M-CSF for 2 days and the the cells were further cultured in M-CSF and RANKL. RNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq.
Results: We detected xxx gense that were higher expression in Dcir-/- OCs, compared with WT OCs, with a fold change ≥1.5 and p value <0.05.
Conclusion: Our data showed that Dcir deficiency in OCs changed the gene expression pattern, compared to WT OCs, which are induced by M-CSF and RANKL.
Overall design mRNA profiles of WT and Dcir-/- osteoclasts for 2 days culture aftre RANKL stimulation
Contributor(s) Shimizu K
Citation(s) 37266426
Submission date Jan 21, 2023
Last update date Jun 07, 2023
Contact name Tomonori Kaifu
Phone +817270212
Organization name Tohoku Medicine and Pharmaceutical University
Department Faculty of Medicine
Lab Division of Immunology
Street address 1-15-1 Fukumuro Miyagino ku
City Sendai
State/province Miyagi
ZIP/Postal code 9838536
Country Japan
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM6951239 WT osteoclasts 3
GSM6951240 WT osteoclasts 82
GSM6951241 WT osteoclasts 94
BioProject PRJNA926132

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Supplementary file Size Download File type/resource
GSE223429_221227result.xlsx 2.7 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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