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| Status |
Public on Sep 01, 2010 |
| Title |
PHF8 mediates histone demethylation events in cell cycle progression [expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
While reversible histone modifications are linked to an ever-expanding range of biological functions, the demethylases for histone H4 lysine 20 and their potential regulatory roles remain unknown. Here, we report that the PHD and Jumonji C (JmjC) domain-containing protein, PHF8, while utilizing multiple substrates, including H3K9me1/2 and H3K27me2, also functions as an H4K20me1 demethylase. PHF8 is recruited to promoters by its PHD domain based on interaction with H3K4me2/3 and controls G1/S transition in conjunction with E2F1, HCF-1 and Set1A, at least in part, by removing the repressive H4K20me1 mark from a subset of E2F1-regulated gene promoters. Phosphorylation-dependent PHF8 dismissal from chromatin in prophase is apparently required for the accumulation of H4K20me1 during early mitosis, which might represent a component of the Condensin II loading process. Accordingly, the HEAT repeat clusters in two non-SMC Condensin II subunits, N-CAPD3 and N-CAPG2, are capable of recognizing H4K20me1, and ChIP-seq. analysis demonstrate a significant overlap of Condensin II and H4K20me1 sites in mitotic HeLa cells. Thus, the identification and characterization of an H4K20me1 demethylase, PHF8, has revealed an intimate link between this enzyme and two distinct events in cell cycle progression.
Keywords: Expression profiling by array
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| Overall design |
HeLa cells were transfected with either control or PHF8 siRNAs. Experiments were performed in biological duplicates. RNA were extracted and sujected to microarray analysis.
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| Citation(s) |
20622854 |
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| Submission date |
Jun 21, 2010 |
| Last update date |
Feb 15, 2013 |
| Contact name |
Michael Rosenfeld |
| E-mail(s) |
mrosenfeld@ucsd.edu
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| Phone |
8585345858
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| Fax |
8585348180
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| URL |
http://rosenfeldlab.ucsd.edu/
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| Organization name |
HHMI/UCSD
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| Department |
School of Medicine
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| Lab |
Michael G Rosenfeld
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL6102 |
Illumina human-6 v2.0 expression beadchip |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE22400 |
PHF8 mediates histone demethylation events in cell cycle progression |
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| Relations |
| BioProject |
PRJNA129263 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22477_RAW.tar |
5.8 Mb |
(http)(custom) |
TAR |
| GSE22477_non-normalized.txt.gz |
7.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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