|
| Status |
Public on Sep 01, 2010 |
| Title |
PHF8siRNA, 1 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa cells
transfection: PHF8siRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Mini Kit (QIAGEN)
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled with biotin using the Ambion Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
hybridization for 18hrs at 58 degree and stained with Streptavidin-Cy3
|
| Scan protocol |
Slides were scanned using the illumina BeadArray Reader scanner
|
| Description |
4343238033B
|
| Data processing |
Raw data was extracted with the Illumina Beadstudio software. The data was processed according to Illumina protocols and normalized using the multi-loess method mloess.
|
| |
|
| Submission date |
Jun 16, 2010 |
| Last update date |
Jun 16, 2010 |
| Contact name |
Michael Rosenfeld |
| E-mail(s) |
mrosenfeld@ucsd.edu
|
| Phone |
8585345858
|
| Fax |
8585348180
|
| URL |
http://rosenfeldlab.ucsd.edu/
|
| Organization name |
HHMI/UCSD
|
| Department |
School of Medicine
|
| Lab |
Michael G Rosenfeld
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL6102 |
| Series (2) |
| GSE22400 |
PHF8 mediates histone demethylation events in cell cycle progression |
| GSE22477 |
PHF8 mediates histone demethylation events in cell cycle progression [expression] |
|
