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| Status |
Public on Dec 31, 2023 |
| Title |
The soil emergence-related transcription factor PIF3 regulates root penetration by interacting with the receptor kinase FER |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The cotyledons of etiolated seedlings from terrestrial flowering plants must emerge from the soil surface, while roots must penetrate the soil to ensure plant survival. We show here that the soil emergence related transcription factor PHYTOCHROME-INTERACTING FACTOR 3 (PIF3) regulates root penetration via transducing external signals perceived by the receptor kinase FERONIA (FER) in Arabidopsis thaliana. The loss of FER function in the fer-4 mutant resulted in a severe defect in root penetration into hard soil or medium. Single-cell RNA-seq profiling of roots revealed a distinct cell clustering pattern, especially for root cap cells, and revealed PIF3 as a putative FER-regulated transcription factor. Biochemical, imaging, and genetic experiments confirmed that PIF3 is required for root soil penetration. Moreover, FER interacted with and stabilized PIF3, which then modulated the expression of mechanosensitive ion channels and the sloughing of outer cells in the root cap. We propose a novel mechanism of soil penetration by plant roots.
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| Overall design |
Col-0 mock, Col-0 RALF1, fer-4 mock and fer-4 RALF1; each group has three replicates; 12 samples in total.
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| Contributor(s) |
Xu F, Li Y |
| Citation missing |
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| Submission date |
Feb 14, 2023 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Fan Xu |
| E-mail(s) |
fan_xu@hnu.edu.cn
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| Organization name |
Hunan University
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| Street address |
N.O. 52 Tianma street
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| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410012 |
| Country |
China |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA934914 |
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