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| Status |
Public on Apr 25, 2023 |
| Title |
Huangqi Decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Purpose: Our study clarifies the mechanism of Huangqi decoction (HQD) against DKD in diabetic db/db mice. Methods: Eight-week-old male diabetic db/db mice were randomly divided into four groups: Model (1% CMC), HQD-L (0.12 g/kg), HQD-M (0.36 g/kg), and HQD-H (1.08 g/kg) groups. Non-diabetic db/m mice were used as a control group. These mice received HQD treatment for 8 weeks continuously. After 8 weeks of feeding, kidneys were harvested to observe the kidney function, pathological changes, micro-assay study, and the protein expression levels. Results: HQD treatment improved the albumin/creatine ratio (ACR) and 24 h urinary albumin, prevented the pathological phenotypes of increased glomerular volume, widened mesangial areas, the proliferation of mesangial matrix, the disappearance of foot processes, the decreased expression of nephrin and the number of podocytes. The expression profile chips were assessed to reveal the global transcriptional response and predict related functions, diseases and pathways. To verify this, we found that HQD treatment activated the protein expressions of BMP1, BMP7, BMPR2, and active-Rap1 and inhibited Smad1 and phospho-ERK. In addition, HQD could improve lipid deposition in the kidneys of db/db mice. Conclusion: HQD prevents the progression of DKD in db/db mice by regulating the transcription of BMPs and their downstream target genes, inhibiting the phosphorylation of ERK and Smad1 by promoting the binding of Rap1 to GTP and regulating the lipid metabolism dysfunction. These provide a new idea for the treatment of DKD.
Overall, HQD had a significant protective effect against DKD. This may be related to the fact that HQD promotes the transcription of BMPs and their downstream target genes by upregulating BMPR-II and regulates the phosphorylation of ERK and Smad by promoting the binding of Rap1 to GTP. In addition, HQD also has a noticeable role in regulating lipid metabolism dysfunction in DKD, which provides a new idea for future research on HQD.
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| Overall design |
Micro-array gene expression analysis was used to identify the DEGs in renal cortex after HQD treatment. The samples (n=3 for each group) with TNA integrity number above 7 were accepted and performed by Wanyen Biotechnologies Inc. (Shanghai).
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| Contributor(s) |
Chen Y, Rong R, Wang L, Wang H, Zhu B, Cao A |
| Citation(s) |
37365530 |
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| Submission date |
Feb 16, 2023 |
| Last update date |
Jul 25, 2023 |
| Contact name |
Aili Cao |
| E-mail(s) |
caoaili0312@sina.cn
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| Phone |
13564273728
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| Organization name |
Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
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| Street address |
600 Yishan Road
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200233 |
| Country |
China |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA935763 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE225477_RAW.tar |
86.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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