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| Status |
Public on Aug 01, 2010 |
| Title |
Transcriptome comparison of B. anthracis Ames and isogenic sinR-null mutant, UTA21 |
| Organism |
Bacillus anthracis |
| Experiment type |
Expression profiling by array
|
| Summary |
To establish the SinR regulon in B. anthracis, gene expression of the fully virulent Ames strain and the isogenic sinR-null strains were compared using expression microarray analysis.
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| Overall design |
Cultures of the two B. anthracis strains tested were grown in triplicate in LB broth to mid-exponential phase (O.D.600 = 0.5-0.6) or early stationary phase of growth (O.D.600 = 3.5-3.9). Cells were pelleted through centrifugation and frozen at -80ºC. Total RNA was isolated using a hot phenol method, converted to cDNA, fragmented, labeled, and hybridized to our Affymetrix microarray. Estimates of gene expression were calculated using GCOS software v1.4.
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| Contributor(s) |
Sumby P |
| Citation(s) |
21131488 |
| |
| Submission date |
Jun 24, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Paul Sumby |
| Organization name |
University of Nevada
|
| Department |
Microbiology & Immunology
|
| Street address |
1554 N. Virginia St.
|
| City |
Reno |
| State/province |
NV |
| ZIP/Postal code |
89557 |
| Country |
USA |
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| Platforms (1) |
| GPL10581 |
Affymetrix Bacillus anthracis custom array_version 2 [B_anthracis] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA128441 |
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