|
| Status |
Public on Aug 01, 2010 |
| Title |
UTA21_expo phase_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Exponential phase
|
| Organism |
Bacillus anthracis |
| Characteristics |
strain: UTA21
growth phase: Exponential phase
|
| Treatment protocol |
6-ml samples were taken in exponential phase and 2-mls samples were collected in stationary phase of growth. Cells were pelleted at 2,400 x g for 10 min at 4ºC and pellets were frozen at -80ºC.
|
| Growth protocol |
Approximately 1x106 B. anthracis spores were inoculated into 25-ml of LB medium and cultures were incubated until mid-exponential (OD600 = 0.5-0.6) or early stationary (OD600 = 3.5-3.9) growth phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a standard hot phenol precipitation.
|
| Label |
Biotin
|
| Label protocol |
RNA was converted to cDNA, the cDNA was fragmented, and the cDNA labeled with biotin
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized to our custom array for 16h at 40ºC. GeneChips were washed and stained using the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using a GeneArray Scanner G2500A.
|
| Description |
UTA21E2
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 50.
|
| |
|
| Submission date |
Jun 24, 2010 |
| Last update date |
Jun 25, 2010 |
| Contact name |
Paul Sumby |
| Organization name |
University of Nevada
|
| Department |
Microbiology & Immunology
|
| Street address |
1554 N. Virginia St.
|
| City |
Reno |
| State/province |
NV |
| ZIP/Postal code |
89557 |
| Country |
USA |
| |
|
| Platform ID |
GPL10581 |
| Series (1) |
| GSE22559 |
Transcriptome comparison of B. anthracis Ames and isogenic sinR-null mutant, UTA21 |
|
