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Series GSE225715 Query DataSets for GSE225715
Status Public on Mar 03, 2023
Title A nucleosome switch primes Hepatitis B Virus infection [Mnase-Seq]
Organisms Homo sapiens; Hepatitis B virus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chronic hepatitis B virus (HBV) infection is an incurable global health threat capable of causing liver disease and hepatocellular carcinoma. During the genesis of infection, HBV establishes an independent chromosome, cccDNA, consisting of the circular viral genome and host histones. The first viral protein expressed, HBx, induces degradation of a host silencing factor to facilitate infection. However, the relationship between cccDNA’s chromatin and early HBx transcription state remains poorly understood. Using reconstituted viral chromosomes, we found that nucleosomes in cccDNA drive HBx transcription. We corroborated these findings in cells and further showed that chromatin destabilizing drugs inhibit viral transcription and antigen expression in hepatocytes. Our results shed new light on a long-standing paradox and represent a novel therapeutic avenue for the treatment of chronic HBV.
Overall design We performed RNA-seq and MNase-seq on HEK293T cells that were transfected with hepatitis B virus (HBV) cccDNA over different timepoitns post transfection. We also performed MNase-seq on HBV cccDNA that was reconstituted with nucleosomes at different levels of saturation.
Contributor(s) Prescott NA, Mansisidor A, Bram Y, Lemmon AA, Lim C, Risca VI, Schwartz RE, David Y
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Submission date Feb 21, 2023
Last update date Mar 05, 2023
Contact name Andres Mansisidor
Organization name Rockefeller University
Department Laboratory of Genome Architecture and Dynamics
Lab Risca
Street address 1230 York Ave Box 176
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (2)
GPL30882 NextSeq 1000 (Homo sapiens)
GPL33150 NextSeq 1000 (Hepatitis B virus)
Samples (27)
GSM7054608 MNase-seq, HBV cccDNA, Empty, In vitro Experiment1
GSM7054609 MNase-seq,HBV cccDNA loaded with reconstituted nucleosomes, In vitro Experiment1
GSM7054610 MNase-seq,HBV dslDNA loaded with reconstituted nucleosomes, In vitro Experiment1
This SubSeries is part of SuperSeries:
GSE225716 A nucleosome switch primes Hepatitis B Virus infection
BioProject PRJNA937215

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Supplementary file Size Download File type/resource
GSE225715_RAW.tar 630.0 Kb (http)(custom) TAR (of BEDGRAPH, TBI)
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Raw data are available in SRA
Processed data provided as supplementary file

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