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| Status |
Public on Apr 14, 2023 |
| Title |
A model for studying homeostatic plasticity in human stem cell derived neuronal networks |
| Organisms |
Homo sapiens; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the functional and molecular effects of neuronal activity suppresion at the network level, we co-cultured human-derived iPSCs neurons with rat astrocytes and treated them with TTX.
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| Overall design |
Transcriptomic profilling of co-cultures of human-derived iPSCs neurons and rat astrocytes treated either with TTX or vehicle. Three biological replicates of vehicle-treated (Veh) and 48 h-TTX treated (TTX) co-cultures (6 samples in total). RNA-seq reads were mapped against the hybrid human and rat reference genome (GRCh38+ Rnor6.0), which allowed to discriminate between human neurons and rat astrocytes-derived transcripts.
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| Contributor(s) |
Yuan X, Puvogel S, van Rhijn J, Esteve-Codina A, Meijer M, Rouschop S, Van Hugte E, Oudakker A, Schoenmaker C, Schubert D, Franke B, Nadif Kasri N |
| Citation(s) |
37863044 |
| |
| Submission date |
Feb 21, 2023 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Sofia Puvogel |
| E-mail(s) |
sofia.puvogel@gmail.com
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| Phone |
+31634357455
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| Organization name |
RadboudUMC
|
| Street address |
Geert Grooteplein Zuid 10
|
| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL27452 |
Illumina NovaSeq 6000 (Homo sapiens; Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA937294 |
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