|
| Status |
Public on Apr 14, 2023 |
| Title |
TTX_2 |
| Sample type |
SRA |
| |
|
| Source name |
hiPSCs-derived neurons/primary culture of rat astrocytes
|
| Organisms |
Homo sapiens; Rattus norvegicus |
| Characteristics |
tissue: hiPSCs-derived neurons/primary culture of rat astrocytes
cell line: iPSCs
cell type: neuron+astrocyte
genotype: WT
treatment: TTX
time: Days in vitro 49
|
| Treatment protocol |
DIV 49 co-cultures were treated with 1 uM TTX for 48 hours
|
| Growth protocol |
Co-cultures of iPSC-derived neurons and rat astrocytes were cultured in neurobasal medium supplemented with B-27, GlutaMAX, Primocin, NT-3, BDNF and doxycycline in humidified atmosphere with 5% CO2 at 37°C until DIV 49
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions.
The RNASeq libraries are prepared with KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche) starting from 500 ng of total RNA
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Multiple_samples_count_matrix_human
Multiple_samples_count_matrix_rat
|
| Data processing |
Mapping : STAR/2.7.8a
Quantification : RSEM/1.3.0
Genome : GRCh38+Rnor6.0
Annotation : gencode39+ensembl104
|
| |
|
| Submission date |
Feb 21, 2023 |
| Last update date |
Apr 14, 2023 |
| Contact name |
Sofia Puvogel |
| E-mail(s) |
sofia.puvogel@gmail.com
|
| Phone |
+31634357455
|
| Organization name |
RadboudUMC
|
| Street address |
Geert Grooteplein Zuid 10
|
| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL27452 |
| Series (1) |
| GSE225761 |
A model for studying homeostatic plasticity in human stem cell derived neuronal networks |
|
| Relations |
| BioSample |
SAMN33401321 |
| SRA |
SRX19463405 |
